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ATG13 Antibody (YA1650)

(Synonyms: Autophagy-related protein 13)
Customer Review

Based on 1 Customer Validation

ATG13 Antibody (YA1650) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG13.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ATG13 Antibody (YA1650)
  • ATG13 Antibody (YA1650) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

ATG13 Antibody (YA1650) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG13.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 72 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 57 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human KIAA0652 aa169-418.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104002

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ATG13 Antibody (YA1650)
    Western blot analysis of extracts from Hela (lane 2, 20ug), MCF-7 (lane 3, 20ug), C6 (lane 4, 20ug) and HepG2 (lane 5, 20ug) using ATG13 Antibody (HY-P81905). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    ATG13 is an Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation

  • Subcellular Localization

    Cytoplasm, cytosol; Preautophagosomal structure

  • Isoforms & Post-Translational Modification

    O75143 has 5 isomers: O75143-1: 56572 Da (predicted); O75143-2: 52823 Da (predicted); O75143-3: 44487 Da (predicted); O75143-4: 44093 Da (predicted); O75143-5: 60155 Da (predicted).
    Phosphorylated by ULK1, ULK2 and mTOR. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. ULK1-mediated phosphorylation of ATG13 at Ser-355 is required for efficient clearance of depolarized mitochondria

  • Subunit

    Part of a complex consisting of ATG13, ULK1 and RB1CC1 (PubMed:19211835, PubMed:19225151, PubMed:19597335, PubMed:24290141). Interacts with ATG101 (PubMed:19287211, PubMed:19597335, PubMed:26299944). Interacts with ULK1 (via C-terminus); this interaction is increased in the absence of TMEM39A (PubMed:18936157, PubMed:19287211, PubMed:21855797, PubMed:31806350). Interacts with ULK2 (via C-terminus) (PubMed:18936157, PubMed:19225151). Interacts (via the LIR motif) with GABARAP, GABARAPL, GABARAPL2 (PubMed:23043107). Interacts (via the LIR motif) with MAP1LC3A, MAP1LC3B and MAP1LC3C (PubMed:24290141). Interacts with TAB2 and TAB3 (PubMed:21976705). Interacts with C9orf72 (PubMed:27334615). Interacts with RB1CC1; this interaction is increased in the absence of TMEM39A (PubMed:31806350)

  • SwissProt ID

    O75143

  • Gene ID
    9776 [NCBI]
  • Synonyms

    Autophagy-related protein 13

  • Research Field

    Cell Biology

ATG13 Antibody (YA1650) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2, 20ug), MCF-7 (lane 3, 20ug), C6 (lane 4, 20ug) and HepG2 (lane 5, 20ug) using ATG13 Antibody (HY-P81905). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • ATG13 Antibody (YA1650) Product Image
    1/16
  • Experimental Validation Results for ATG13 Antibody (YA1650)
  • ATG13 Antibody (YA1650) Product Image
  • Experimental Validation Results for ATG13 Antibody (YA1650)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2, 20ug), MCF-7 (lane 3, 20ug), C6 (lane 4, 20ug) and HepG2 (lane 5, 20ug) using ATG13 Antibody (HY-P81905). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ATG13 Antibody (YA1650)
  • ATG13 Antibody (YA1650) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2, 20ug), MCF-7 (lane 3, 20ug), C6 (lane 4, 20ug) and HepG2 (lane 5, 20ug) using ATG13 Antibody (HY-P81905). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • ATG13 Antibody (YA1650) Product Image WB