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Flotillin-1 Antibody (YA760)(PBS only)

(Synonyms: Flotillin-1)

Flotillin-1 Antibody (YA760) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Flotillin-1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Flotillin-1 Antibody (YA760)(PBS only)
  • Flotillin-1 Antibody (YA760)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Flotillin-1 Antibody (YA760) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Flotillin-1.

  • Host Mouse
  • Species Reactivity
    Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 47 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Immunogen

Synthetic peptide corresponding to Flotillin-1.The exact sequence is proprietary to MCE.

Purification

affinity purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Flotillin-1 Antibody (YA760)(PBS only)
    Western blot analysis of extracts from Neuro-2a(lane 2(20ug) and Neuro-2a(lane 3(40ug) using Flotillin 1 Antibody (HY-P80675) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Flotillin-1 may act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles

  • Subcellular Localization

    Cell membrane; Peripheral membrane protein; Endosome; Membrane, caveola; Peripheral membrane protein; Melanosome; Membrane raft

  • Isoforms & Post-Translational Modification

    O75955 has 2 isomers: O75955-1: 47355 Da (predicted); O75955-2: 42080 Da (predicted).

  • Subunit

    Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2 (By similarity). Interacts with ECPAS

  • SwissProt ID

    O75955

  • Gene ID
    10211 [NCBI]
  • Synonyms

    Flotillin-1

  • Research Field

    Cell Biology

Flotillin-1 Antibody (YA760)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Neuro-2a(lane 2(20ug) and Neuro-2a(lane 3(40ug) using Flotillin 1 Antibody (HY-P80675) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Flotillin-1 Antibody (YA760)(PBS only) Product Image
    1/16
  • Experimental Validation Results for Flotillin-1 Antibody (YA760)(PBS only)
  • Flotillin-1 Antibody (YA760)(PBS only) Product Image
  • Experimental Validation Results for Flotillin-1 Antibody (YA760)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Neuro-2a(lane 2(20ug) and Neuro-2a(lane 3(40ug) using Flotillin 1 Antibody (HY-P80675) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Flotillin-1 Antibody (YA760)(PBS only)
  • Flotillin-1 Antibody (YA760)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Neuro-2a(lane 2(20ug) and Neuro-2a(lane 3(40ug) using Flotillin 1 Antibody (HY-P80675) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Flotillin-1 Antibody (YA760)(PBS only) Product Image WB