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eIF1A Antibody (YA466)

(Synonyms: EIF1A; EIF4C; eIF-1A; eIF-4C; EIF1AP1)
Customer Review

Based on 1 Customer Validation

eIF1A Antibody (YA466) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to eIF1A.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for eIF1A Antibody (YA466)
  • Experimental Validation Results for eIF1A Antibody (YA466)
  • eIF1A Antibody (YA466) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200 1:20

Product Details

Description

eIF1A Antibody (YA466) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to eIF1A.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 16 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 16 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human eIF1A aa97-110.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102152

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for eIF1A Antibody (YA466)
    Immunocytochemistry analysis of Hela cells labeling eIF1A with eIF1A Antibody (HY-P80649) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with eIF1A Antibody (HY-P80649) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for eIF1A Antibody (YA466)
    Immunocytochemistry analysis of HepG2 cells labeling eIF1A with eIF1A Antibody (HY-P80649) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with eIF1A Antibody (HY-P80649) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    eIF1A is a conserved eukaryotic translation initiation factor that binds the 40S ribosomal subunit and supports ternary-complex loading, mRNA scanning, start-codon selection, and subunit joining[1]. Mechanistically, eIF1A and eIF1 promote an open, scanning-competent preinitiation complex, which closes after AUG recognition and eIF1 release[2]. Structural studies show that the eIF1A N-terminal tail stabilizes the codon-anticodon duplex in the closed 48S complex, linking eIF1A directly to translation initiation fidelity[3]. eIF1A also coordinates with eIF5 during start-codon recognition, because movement of the eIF1A C-terminal tail toward eIF5 helps trigger phosphate release from eIF2[4]. In cancer-relevant models, EIF1AX mutations affecting the eIF1A N-terminal tail are associated with uveal melanoma and increase discrimination against poor initiation sites in yeast systems[5]. Compared with related initiation factors, eIF1A shows distinct tail-dependent roles: its N-terminal tail can stabilize initiation-site selection, whereas its C-terminal tail promotes scanning and regulates commitment to initiation[1][5]. For experimental applications, inhibitors of the eIF1A-ribosome interaction revealed uORF-dependent translation reinitiation and reduced ovarian-cancer xenograft growth without apparent toxicity[6].

  • Subcellular Localization

    Cytoplasm

  • Subunit

    Component of the 43S pre-initiation complex (43S PIC), which is composed of the 40S ribosomal subunit, EIF1, eIF1A (EIF1AX), eIF3 complex, EIF5 and eIF2-GTP-initiator tRNA complex (eIF2 ternary complex). Interacts with EIF5; this interaction contributes to the maintenance of EIF1 within the open 43S PIC (PubMed:24319994). Interacts through its C-terminal domain (CTD) with the CTD of EIF5B; from the location of the start codon by the 43S complex until the formation of the 80S complex (PubMed:12569173, PubMed:30211544, PubMed:35732735)

  • SwissProt ID

    P47813

  • Gene ID
    1964 [NCBI]
  • Synonyms

    EIF1A; EIF4C; eIF-1A; eIF-4C; EIF1AP1

  • Research Field

    Epigenetics and Nuclear Signaling

[1]. Yu Y, et al. Position of eukaryotic translation initiation factor eIF1A on the 40S ribosomal subunit mapped by directed hydroxyl radical probing. Nucleic Acids Res. 2009 Aug;37(15):5167-82. [Content Brief]

[2]. Passmore LA, et al. The eukaryotic translation initiation factors eIF1 and eIF1A induce an open conformation of the 40S ribosome. Mol Cell. 2007 Apr 13;26(1):41-50. [Content Brief]

[3]. Hussain T, et al. Structural changes enable start codon recognition by the eukaryotic translation initiation complex. Cell. 2014 Oct 23;159(3):597-607. [Content Brief]

[4]. Nanda JS, et al. Coordinated movements of eukaryotic translation initiation factors eIF1, eIF1A, and eIF5 trigger phosphate release from eIF2 in response to start codon recognition by the ribosomal preinitiation complex. J Biol Chem. 2013 Feb 22;288(8):5316-29. [Content Brief]

[5]. Martin-Marcos P, et al. eIF1A residues implicated in cancer stabilize translation preinitiation complexes and favor suboptimal initiation sites in yeast. Elife. 2017 Dec 5;6:e31250. [Content Brief]

[6]. Hayat D, et al. Inhibitors of eIF1A-ribosome interaction unveil uORF-dependent regulation of translation initiation and antitumor and antiviral effects. EMBO J. 2025 Jul;44(13):3853-3871. [Content Brief]

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eIF1A Antibody (YA466) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling eIF1A with eIF1A Antibody (HY-P80649) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with eIF1A Antibody (HY-P80649) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling eIF1A with eIF1A Antibody (HY-P80649) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with eIF1A Antibody (HY-P80649) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • eIF1A Antibody (YA466) Product Image
    1/16
  • Experimental Validation Results for eIF1A Antibody (YA466)
  • Experimental Validation Results for eIF1A Antibody (YA466)
  • eIF1A Antibody (YA466) Product Image
  • Experimental Validation Results for eIF1A Antibody (YA466)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling eIF1A with eIF1A Antibody (HY-P80649) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with eIF1A Antibody (HY-P80649) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for eIF1A Antibody (YA466)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling eIF1A with eIF1A Antibody (HY-P80649) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with eIF1A Antibody (HY-P80649) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for eIF1A Antibody (YA466)
  • Experimental Validation Results for eIF1A Antibody (YA466)
  • eIF1A Antibody (YA466) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling eIF1A with eIF1A Antibody (HY-P80649) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with eIF1A Antibody (HY-P80649) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • eIF1A Antibody (YA466) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling eIF1A with eIF1A Antibody (HY-P80649) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with eIF1A Antibody (HY-P80649) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • eIF1A Antibody (YA466) Product Image ICC
  • eIF1A Antibody (YA466) Product Image ICC