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ATG9A Antibody (YA3081)

(Synonyms: ATG9A; APG9-like 1; Autophagy 9-like 1 protein; Autophagy-related protein 9A; MGD3208; MATG9; APG9 autophagy 9-like 1; APG9L1; Autophagy related 9A)
Customer Review

Based on 1 Customer Validation

ATG9A Antibody (YA3081) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG9A.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for ATG9A Antibody (YA3081)
  • Experimental Validation Results for ATG9A Antibody (YA3081)
  • Experimental Validation Results for ATG9A Antibody (YA3081)
  • ATG9A Antibody (YA3081) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50

Product Details

Description

ATG9A Antibody (YA3081) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG9A.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 94 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 94 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human ATG9A aa812-839.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105449

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ATG9A Antibody (YA3081)
    Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using ATG9A Antibody (HY-P83336) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ATG9A Antibody (YA3081)
    Immunocytochemistry analysis of Hela cells labeling ATG9A with ATG9A Antibody (HY-P83336) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ATG9A Antibody (HY-P83336) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ATG9A Antibody (YA3081)
    Immunocytochemistry analysis of HepG2 cells labeling ATG9A with ATG9A Antibody (HY-P83336) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ATG9A Antibody (HY-P83336) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    ATG9A is a Phospholipid scramblase involved in autophagy by mediating autophagosomal membrane expansion. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 (ATG2A or ATG2B) from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion. Also required to supply phosphatidylinositol 4-phosphate to the autophagosome initiation site by recruiting the phosphatidylinositol 4-kinase beta (PI4KB) in a process dependent on ARFIP2, but not ARFIP1. In addition to autophagy, also plays a role in necrotic cell death[1][2][3][4][5][6][7][8][9][10].

  • Subcellular Localization

    Preautophagosomal structure membrane; Multi-pass membrane protein; Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein; Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein; Late endosome membrane; Multi-pass membrane protein; Recycling endosome membrane; Multi-pass membrane protein; Endoplasmic reticulum membrane; Multi-pass membrane protein; Mitochondrion membrane; Multi-pass membrane protein

  • Isoforms & Post-Translational Modification

    Q7Z3C6 has 3 isomers: Q7Z3C6-1: 94447 Da (predicted); Q7Z3C6-2: 87379 Da (predicted); Q7Z3C6-3: 60694 Da (predicted).
    Ufmylated in a DDRGK1 dependent manner

  • Subunit

    Homotrimer; forms a homotrimer with a central pore that forms a path between the two membrane leaflets (PubMed:32610138, PubMed:33106659). Interacts (via cytoplasmic its C-terminus) with ATG2A (PubMed:32610138, PubMed:33106659). Interacts with SUPT20H (PubMed:19893488). Interacts (via the tyrosine-based sorting signal motif) with AP4M1; promoting association with the AP-4 complex (PubMed:29180427). Interacts with ARFIP1 and ARFIP2 (PubMed:30917996). Interacts with PI4K2A and PI4KB (PubMed:30917996). Interacts with ATG4A; the interaction is direct and promotes ATG9A trafficking (PubMed:33773106)

  • SwissProt ID

    Q7Z3C6

  • Gene ID
    79065 [NCBI]
  • Synonyms

    ATG9A; APG9-like 1; Autophagy 9-like 1 protein; Autophagy-related protein 9A; MGD3208; MATG9; APG9 autophagy 9-like 1; APG9L1; Autophagy related 9A

  • Research Field

    Cell Biology

ATG9A Antibody (YA3081) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using ATG9A Antibody (HY-P83336) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ATG9A with ATG9A Antibody (HY-P83336) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ATG9A Antibody (HY-P83336) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling ATG9A with ATG9A Antibody (HY-P83336) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ATG9A Antibody (HY-P83336) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ATG9A Antibody (YA3081) Product Image
    1/16
  • Experimental Validation Results for ATG9A Antibody (YA3081)
  • Experimental Validation Results for ATG9A Antibody (YA3081)
  • Experimental Validation Results for ATG9A Antibody (YA3081)
  • ATG9A Antibody (YA3081) Product Image
  • Experimental Validation Results for ATG9A Antibody (YA3081)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using ATG9A Antibody (HY-P83336) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ATG9A Antibody (YA3081)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ATG9A with ATG9A Antibody (HY-P83336) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ATG9A Antibody (HY-P83336) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ATG9A Antibody (YA3081)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling ATG9A with ATG9A Antibody (HY-P83336) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ATG9A Antibody (HY-P83336) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ATG9A Antibody (YA3081)
  • Experimental Validation Results for ATG9A Antibody (YA3081)
  • Experimental Validation Results for ATG9A Antibody (YA3081)
  • ATG9A Antibody (YA3081) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HepG2(lane 2(20ug) and HepG2(lane 3(40ug) using ATG9A Antibody (HY-P83336) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ATG9A Antibody (YA3081) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling ATG9A with ATG9A Antibody (HY-P83336) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ATG9A Antibody (HY-P83336) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ATG9A Antibody (YA3081) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling ATG9A with ATG9A Antibody (HY-P83336) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ATG9A Antibody (HY-P83336) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ATG9A Antibody (YA3081) Product Image WB
  • ATG9A Antibody (YA3081) Product Image ICC
  • ATG9A Antibody (YA3081) Product Image ICC