NF2 Antibody (YA1386)(PBS only)

Cat. No.: HY-P81641A: Data Sheet User Guide for Antibodies
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NF2 Antibody (YA1386) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NF2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

NF2 Antibody (YA1386) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NF2.

Host

Rabbit

Molecular Weight

Predicted band size: 70 kDa;

Observed band size: 70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P35240

Gene ID

4771 [NCBI]

Immunogen

A synthetic peptide of human NF2/Merlin

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex

Subcellular Localization：Cell projection, filopodium membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side; Nucleus; Cytoplasm, perinuclear region; Cytoplasmic granule; Cytoplasm, perinuclear region; Cytoplasmic granule; Nucleus; Cell projection, filopodium membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side; Cytoplasm, perinuclear region; Cytoplasmic granule; Cytoplasm, cytoskeleton

Expression：
Tissue_specificity:Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in human tissues, with the exception of human retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels

Isoforms & Post-Translational Modification：P35240 has 10 isomers: P35240-1: 69690 Da (predicted); P35240-2: 72514 Da (predicted); P35240-3: 69090 Da (predicted); P35240-4: 59096 Da (predicted); P35240-5: 64189 Da (predicted); P35240-6: 63996 Da (predicted); P35240-7: 30454 Da (predicted); P35240-8: 65350 Da (predicted); P35240-9: 19215 Da (predicted); P35240-10: 24515 Da (predicted).
Phosphorylation of Ser-518 inhibits nuclear localization by disrupting the intramolecular association of the FERM domain with the C-terminal tail (PubMed:20178741). The dephosphorylation of Ser-518 favors the interaction with NOP53 (PubMed:21167305);Ubiquitinated by the CUL4A-RBX1-DDB1-DCAF1/VprBP E3 ubiquitin-protein ligase complex for ubiquitination and subsequent proteasome-dependent degradation

Subunit：Interacts with NHERF1, HGS and AGAP2. Interacts with LAYN (By similarity). Interacts with SGSM3. Interacts (via FERM domain) with MPP1. Interacts with WWC1. Interacts with the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex. The unphosphorylated form interacts (via FERM domain) with VPRBP/DCAF1. Interacts (via FERM domain) with NOP53; the interaction is direct (PubMed:21167305). Interacts with SCHIP1; the interaction is direct (PubMed:10669747)

Research Field

Neuroscience

Synonyms

ACN; BANF; Merlin; Moesin ezrin radixin like protein; Neurofibromatosis 2; Neurofibromin 2; Nf2; SCH; Schwannomerlin

Documentation

User Guide for Antibodies (1077 KB)