2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies
  5. eNOS Antibody (YA4993)

eNOS Antibody (YA4993) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to eNOS.

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容量 在庫状況
50 μL   お問い合わせ  
100 μL   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 説明

製品説明

eNOS Antibody (YA4993) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to eNOS.
Host

Mouse
Clonality

Monoclonal
分子量

Predicted band size: 133 kDa;
Species Reactivity
Mouse, Rat Predicted Reactivity: Human
Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
SwissProt ID
Gene ID
Immunogen

KLH conjugated synthetic peptide derived from human eNos. The exact sequence is proprietary to MCE.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:100-500
IHC-F
IHC-F: Immunohistochemistry-Frozen
1:100-500
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
1:100-500
Sensitivity Endogenous 純度 affinity purified by Protein G
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
輸送条件

Shipping with blue ice.
Background
Function：Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets; Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1
Subcellular Localization：Cell membrane; Membrane, caveola; Cytoplasm, cytoskeleton; Golgi apparatus
Expression：
Tissue_specificity:Platelets, placenta, liver and kidneys
Isoforms & Post-Translational Modification：P29474 has 3 isomers: P29474-1: 133275 Da (predicted); P29474-2: 68965 Da (predicted); P29474-3: 67862 Da (predicted).
Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity
Subunit：Homodimer. Interacts with NOSIP and NOSTRIN (PubMed:11149895, PubMed:12446846). Interacts with HSP90AB1 (By similarity) (PubMed:11149895, PubMed:12446846, PubMed:23585225). Forms a complex with ASL, ASS1 and SLC7A1; the complex regulates cell-autonomous L-arginine synthesis and citrulline recycling while channeling extracellular L-arginine to nitric oxide synthesis pathway (By similarity)
Synonyms
NOS3; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
ドキュメンテーション
User Guide for Antibodies (1077 KB)

eNOS Antibody (YA4993) 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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製品名:
eNOS Antibody (YA4993)
製品番号:
HY-P85301
数量:
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