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CD74 Antibody (YA5590)

(Synonyms: CD 74 antibody; CD74 antibody; CD74 antigen; invariant polypeptide of major histocompatibility complex; class II antigen-associated; antibody; CD74 antigen antibody; CD74 molecule antibody; CD74 molecule; major histocompatibility complex; class II invariant chain antibody; CLIP antibody; DHLAG antibody; Gamma chain of class II antigens antibody; HG2A_HUMAN antibody; HLA class II histocompatibility antigen gamma chain antibody; HLA DR antigens associated invariant chain antibody; HLA DR gamma antibody; HLA-DR antigens-associated invariant chain antibody; HLA-DR-gamma antibody; HLADG antibody; HLADR antigens associated invariant chain antibody; Ia antigen associated invariant chain antibody; Ia antigen-associated invariant chain antibody; Ia associated invariant chain antibody; Ia gamma antibody; Ii antibody; Invariant polypeptide of major histocompatibility complex class II antigen associated antibody; la-gamma antibody; Major histocompatibility complex class II invariant chain antibody; MHC HLA DR gamma chain antibody; MHC HLA-DR gamma chain antibody; p33 antibody; p35 antibody; Protein 41 antibody; )
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Based on 1 publication(s) in Google Scholar

CD74 Antibody (YA5590) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CD74.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    IHC-P, WB, ICC/IF, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD74 Antibody (YA5590)
  • CD74 Antibody (YA5590) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1000 1:500-2000 1:100-500 1:1000-5000

Product Details

Description

CD74 Antibody (YA5590) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CD74.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 33 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 34 kDa
Immunogen

Synthesized peptide derived from human CD74 AA range: 150-250

Purification

Protein G

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD74 Antibody (YA5590)
    Western blot analysis of extracts from Daudi (lane2, 20μg), Raji (lane3, 20μg), LOVO (lane4, 20μg) and Jurkat (lane5, 20μg) using CD74 Antibody (HY-P85898). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    CD74 (HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain) is a type II transmembrane protein. CD74 is involved in the formation and transport of MHC class II peptide complexes for the generation of CD4+ T cell responses. Cell surface CD74 serves as a receptor for the cytokine, macrophage migration inhibitory factor (MIF) on many cell types. In immune cells, MIF binding to CD74 induces a signaling cascade that results in regulation of cell proliferation and survival. CD74 has been associated with tumor progression and metastasis. CD74 expression has been suggested to serve as a prognostic factor in many cancers, with higher relative expression behaving as a marker of tumor progression[1].

  • Subcellular Localization

    Cell membrane; Single-pass type II membrane protein; Endoplasmic reticulum membrane; Golgi apparatus, trans-Golgi network; Endosome; Lysosome; Secreted; Late endosome; Lysosome

  • Expression


    Tissue_specificity:Protein levels are detectable in urine; it accounts for 10% of total CD74 expression in B cells and 70% of total CD74 expression in other B cells.

  • Isoforms & Post-Translational Modification

    P04233 has 5 isomers: P04233-1: 33516 Da (predicted); P04233-2: 26399 Da (predicted); P04233-3: 18328 Da (predicted); P04233-4: 31492 Da (predicted); P04233-5: 24375 Da (predicted).
    O-glycosylated with core 1 or possibly core 8 glycans (PubMed:22171320, PubMed:23234360). Contains chondroitin sulfate (PubMed:25326458)

  • Subunit

    Homotrimer. In the endoplasmic reticulum (ER) it forms a heterononameric MHC II-Ii complex: 3 MHC class II molecules (heterodimers of an alpha and a beta subunit) bind to the CD74 homotrimer (also known as invariant chain or HLA class II histocompatibility antigen gamma chain). In the endosomal/lysosomal system, the CD74 component undergoes sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide) attached to the MHC class II molecule (alpha-beta-CLIP complex). This processed complex interacts with HLA_DM and HLA_DO heterodimers in order to release CLIP and facilitate the binding of antigenic peptides to the MHC class II molecules. Interacts with CD44; this complex is essential for the MIF-induced signaling cascade that results in B cell survival

  • SwissProt ID

    P04233

  • Gene ID
    972 [NCBI]
  • Synonyms

    CD 74 antibody; CD74 antibody; CD74 antigen; invariant polypeptide of major histocompatibility complex; class II antigen-associated; antibody; CD74 antigen antibody; CD74 molecule antibody; CD74 molecule; major histocompatibility complex; class II invariant chain antibody; CLIP antibody; DHLAG antibody; Gamma chain of class II antigens antibody; HG2A_HUMAN antibody; HLA class II histocompatibility antigen gamma chain antibody; HLA DR antigens associated invariant chain antibody; HLA DR gamma antibody; HLA-DR antigens-associated invariant chain antibody; HLA-DR-gamma antibody; HLADG antibody; HLADR antigens associated invariant chain antibody; Ia antigen associated invariant chain antibody; Ia antigen-associated invariant chain antibody; Ia associated invariant chain antibody; Ia gamma antibody; Ii antibody; Invariant polypeptide of major histocompatibility complex class II antigen associated antibody; la-gamma antibody; Major histocompatibility complex class II invariant chain antibody; MHC HLA DR gamma chain antibody; MHC HLA-DR gamma chain antibody; p33 antibody; p35 antibody; Protein 41 antibody;

[1]. Gil-Yarom N, et al. CD74 is a novel transcription regulator. Proc Natl Acad Sci U S A. 2017 Jan 17;114(3):562-567. [Content Brief]

CD74 Antibody (YA5590) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Daudi (lane2, 20μg), Raji (lane3, 20μg), LOVO (lane4, 20μg) and Jurkat (lane5, 20μg) using CD74 Antibody (HY-P85898). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • CD74 Antibody (YA5590) Product Image
    1/16
  • Experimental Validation Results for CD74 Antibody (YA5590)
  • CD74 Antibody (YA5590) Product Image
  • Experimental Validation Results for CD74 Antibody (YA5590)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Daudi (lane2, 20μg), Raji (lane3, 20μg), LOVO (lane4, 20μg) and Jurkat (lane5, 20μg) using CD74 Antibody (HY-P85898). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD74 Antibody (YA5590)
  • CD74 Antibody (YA5590) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Daudi (lane2, 20μg), Raji (lane3, 20μg), LOVO (lane4, 20μg) and Jurkat (lane5, 20μg) using CD74 Antibody (HY-P85898). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • CD74 Antibody (YA5590) Product Image WB