PPM1A Antibody (YA3864)

Art. -Nr.: HY-P84167: Data Sheet COA
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User Guide for Antibodies
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PPM1A Antibody (YA3864) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PPM1A.

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Größe	Verfügbarkeit	Menge
20 μL	Auf Lager	Estimated Time of Arrival: December 31
50 μL	Auf Lager	Estimated Time of Arrival: December 31
100 μL	Auf Lager	Estimated Time of Arrival: December 31
250 μL	Angebot einholen

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Beschreibung

Beschreibung

PPM1A Antibody (YA3864) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PPM1A.

Host

Mouse

Clonality

Monoclonal

Molekulargewicht

Predicted band size: 42 kDa;

Observed band size: 42 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Monkey

SwissProt ID

P20265

Gene ID

5494 [NCBI]

Immunogen

Purified recombinant fragment of human PPM1A (AA: 202-382) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Reinheit	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Verification Image

ALL WB FC

Western blot analysis of extracts from COS-7(lane 1(40μg) ),MCF-7(lane 2(40μg) ),HepG2(lane 3(40μg) ),HEK293(lane 4(40μg) ),Hela(lane 5(40μg) )and A431(lane 6(40μg) ) using PPM1A antibody(30376P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
Flow cytometric analysis of 1X10^6 Hela cells labeling PPM1A Antibody(green). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (gray) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (red).

Background

Function：Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (By similarity). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:Specifically expressed in neuroectodermal cell lineages

Subunit：Interacts with PQBP1 (PubMed:10332029). Interaction with ISL1 (By similarity)

Synonyms

PP2CA; PP2Calpha; PP2C-ALPHA

Dokumentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)