KLHL2 Antibody (YA8500)

Cat. No.: HY-P88816: Ficha de datos User Guide for Antibodies
FAQs
Technical Support

KLHL2 Antibody (YA8500) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to KLHL2.

Para uso exclusivo en investigación. No vendemos a pacientes.

Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

* Seleccione Cantidad antes de agregar artículos.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn
Referencias

Descripciòn

KLHL2 Antibody (YA8500) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to KLHL2.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 65.8 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O95198

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-100 and 494-593 of human KLHL2 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Pureza	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2b

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that mediates the ubiquitination of target proteins, such as NPTXR, WNK1, WNK3 and WNK4, leading most often to their proteasomal degradation (PubMed:23838290). The BCR(KLHL2) complex catalyzes ubiquitination and degradation of NPTXR (By similarity). Responsible for degradative ubiquitination of the WNK kinases WNK1, WNK3 and WNK4 (PubMed:23838290). Plays a role in the reorganization of the actin cytoskeleton (PubMed:10397770). Promotes growth of cell projections in oligodendrocyte precursors (PubMed:15715669)

Subcellular Localization：Cytoplasm, cytoskeleton,Cell projection, ruffle,Cell projection,Cell projection, lamellipodium,Cytoplasm, cytosol

Expression：
Tissue_Specificity: Ubiquitous. Detected throughout the brain

Isoforms & Post-Translational Modification：O95198 has three isomers: O95198-1: 65975 Da (predicted); O95198-2: 66624 Da (predicted); O95198-3: 56086 Da (predicted).

Subunit：Component of the BCR(KLHL2) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL2 and RBX1 (PubMed:23838290)

Synonyms

ABP-KELCH; MAV; MAYVEN

Documentación

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/baf3-cell.html [Content Brief]