Perilipin-1 Antibody (YA6063)

Cat. No.: HY-P86371: Fiche technique COA
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Perilipin-1 Antibody (YA6063) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Perilipin-1.

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Size	Stock	Quantité
20 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtenir un devis

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Description
Références

Description

Perilipin-1 Antibody (YA6063) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Perilipin-1.

Host

Rabbit

Clonality

Monoclonal

Masse moléculaire

Predicted band size: 56 kDa;

Observed band size: 56 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O60240

Gene ID

5346 [NCBI]

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:400-1:1000
WB WB: Western Blot	1:2000-1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000
IP IP: Immunoprecipitation	1:50-1:200

Pureté	Protein A	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Verification Image

IHC-P

Immunohistochemical analysis of paraffin-embedded human breast tissue using Perilipin-1 Antibody (YA6063). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86371, 1/150) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human breast tissue using Perilipin-1 Antibody (YA6063). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86371, 1/150) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human fatty liver disease tissue using Perilipin-1 Antibody (YA6063). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86371, 1/150) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human fatty liver disease tissue using Perilipin-1 Antibody (YA6063). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86371, 1/150) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human skin tissue using Perilipin-1 Antibody (YA6063). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86371, 1/150) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human skin tissue using Perilipin-1 Antibody (YA6063). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86371, 1/150) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human liposarcoma tissue using Perilipin-1 Antibody (YA6063). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86371, 1/150) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human liposarcoma tissue using Perilipin-1 Antibody (YA6063). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86371, 1/150) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

Background

Function：Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels

Subcellular Localization：Endoplasmic reticulum; Lipid droplet

Expression：
Tissue_specificity:Protein levels were detected in adipocytes of white adipose tissue (PubMed: 27832861) . It was also detected in visceral adipose tissue and mammary glands (PubMed: 9521880) .

Subunit：Interacts with ABHD5 (By similarity). Interacts with CIDEC (PubMed:23399566). Interacts with AQP7 (PubMed:27832861)

RRID

AB_3719136

Documentation

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Fiche technique (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Références

[1]. /biology-dictionary/pc-3-cell.html [Content Brief]