RRM2 Antibody (YA1885)

Cat. No.: HY-P82140: Data Sheet COA
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User Guide for Antibodies
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RRM2 Antibody (YA1885) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RRM2.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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RRM2 Antibody (YA1885) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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References

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RRM2 Antibody (YA1885) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RRM2.

Host

Rabbit

Clonality

Recombinant, Monoclonal

분자량

Predicted band size: 45 kDa;

Observed band size: 45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P31350

Gene ID

6241 [NCBI]

Immunogen

Recombinant protein of human RRM2

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), HepG2 (lane 2, 20 μg), K562 (lane 3, 20 μg), Jurkat (lane 4, 20 μg), A431 (lane 5, 20 μg), and HUVEC (lane 6, 20 μg) using RRM2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Induction:Up-regulated in response to DNA damage induced by doxorubicin, camptothecin, UV-C, methyl methanesulfonate, nocodazole, or gamma-irradiation

Isoforms & Post-Translational Modification：P31350 has 2 isomers: P31350-1: 44878 Da (predicted); P31350-2: 51093 Da (predicted).
Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling. Phosphorylated on Thr-33 by CDK1 and CDK2; predominantly in G2 and M phase (PubMed:22632967);Ubiquitinated by the SCF(CCNF) E3 ubiquitin-protein ligase complex; leading to its degradation by the proteasome

Subunit：Heterodimer of a large and a small subunit. Interacts (via Cy motif and when phosphorylated at Thr-33) with CCNF; the interaction occurs exclusively in G2 and early M (PubMed:22632967)

RRID

AB_3104254

Database

Entrez Gene: 6241 Human

SwissProt: P31350 Human

OMIM: 180390 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

R2; RR2M

각종 서류

Select Batch:

Data Sheet (258 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/hct-116-cell.html [Content Brief]

RRM2 Antibody (YA1885) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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RRM2 Antibody (YA1885)

RRM2 Antibody (YA1885) Featured Recommendations:

Product Detail

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Background

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RRM2 Antibody (YA1885) Related Classifications

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