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NADPH Oxidase 4 Antibody (YA4640)

(Synonyms: NADPH oxidase 4; Kidney oxidase-1; KOX-1; KOX1; Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase; NOX4; RENOX)
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Based on 1 publication(s) in Google Scholar

NADPH Oxidase 4 Antibody (YA4640) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NADPH Oxidase 4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • NADPH Oxidase 4 Antibody (YA4640) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:20

Product Details

Description

NADPH Oxidase 4 Antibody (YA4640) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NADPH Oxidase 4.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 67 kDa
Species Reactivity Database

Entrez Gene: 50507 Human

SwissProt: Q9NPH5 Human

Immunogen

Synthetic peptide corresponding to Human NOX4 aa510-578.

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718727

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
    Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), JAR (lane 2, 15 μg), and U-87MG (lane 3, 15 μg) using NADPH Oxidase 4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
    Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
    Immunocytochemistry analysis of Hela cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P80762A) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P80762A) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
    Immunocytochemistry analysis of HepG2 cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P80762A) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P80762A) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    NADPH Oxidase 4 is a NADPH oxidase that catalyzes predominantly the reduction of oxygen to H2O2. Can also catalyze to a smaller extent, the reduction of oxygen to superoxide. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Promotes ferroptosis, reactive oxygen species production and reduced glutathione (GSH) levels by activating NLRP3 inflammasome activation and cytokine release; NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. Involved in redox signaling in vascular cells. Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage; Lacks superoxide-generating NADPH oxidase activity[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16].

  • Subcellular Localization

    Cytoplasm; Endoplasmic reticulum membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein; Cell junction, focal adhesion; Nucleus; Nucleus; Nucleus, nucleolus; Cytoplasm; Cytoplasm, perinuclear region; Cytoplasm; Cytoplasm, perinuclear region; Cytoplasm; Cytoplasm, perinuclear region; Cytoplasm; Cytoplasm, perinuclear region

  • Expression


    Tissue_specificity:This gene is expressed (protein level) in distal renal tubular cells and endothelial cells of the renal cortex. Expression is widespread. It is highly expressed in the kidneys, and expressed at lower levels in the heart, adipocytes, liver cancer cells, endothelial cells, skeletal muscle, brain, various brain tumor cell lines, and airway epithelial cells.

    Induction:By 7-ketocholesterol (at protein level)

  • Isoforms & Post-Translational Modification

    Q9NPH5 has 9 isomers: Q9NPH5-1: 66932 Da (predicted); Q9NPH5-2: 58410 Da (predicted); Q9NPH5-3: 27625 Da (predicted); Q9NPH5-4: 31812 Da (predicted); Q9NPH5-5: 25761 Da (predicted); Q9NPH5-6: 62745 Da (predicted); Q9NPH5-7: 6516 Da (predicted); Q9NPH5-8: 64102 Da (predicted); Q9NPH5-9: 59915 Da (predicted).
    Deubiquitinated by USP19;N-glycosylated and glycosylation is required for its proper function;N-glycosylated

  • Subunit

    Interacts with protein disulfide isomerase (By similarity). Interacts with, relocalizes and stabilizes CYBA/p22phox. Interacts with TLR4. Interacts with PPP1R15A (PubMed:26742780). Interacts with LRRC8A; this interaction prevents the ubiquitin-mediated degradation of LRRC8A (PubMed:39909992)

  • SwissProt ID

    Q9NPH5

  • Gene ID
    50507 [NCBI]
  • Synonyms

    NADPH oxidase 4; Kidney oxidase-1; KOX-1; KOX1; Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase; NOX4; RENOX

  • Research Field

    Cardiovascular

NADPH Oxidase 4 Antibody (YA4640) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), JAR (lane 2, 15 μg), and U-87MG (lane 3, 15 μg) using NADPH Oxidase 4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P80762A) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P80762A) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P80762A) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P80762A) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NADPH Oxidase 4 Antibody (YA4640) Product Image
    1/16
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • NADPH Oxidase 4 Antibody (YA4640) Product Image
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), JAR (lane 2, 15 μg), and U-87MG (lane 3, 15 μg) using NADPH Oxidase 4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P80762A) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P80762A) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P80762A) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P80762A) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • Experimental Validation Results for NADPH Oxidase 4 Antibody (YA4640)
  • NADPH Oxidase 4 Antibody (YA4640) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), JAR (lane 2, 15 μg), and U-87MG (lane 3, 15 μg) using NADPH Oxidase 4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • NADPH Oxidase 4 Antibody (YA4640) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • NADPH Oxidase 4 Antibody (YA4640) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • NADPH Oxidase 4 Antibody (YA4640) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • NADPH Oxidase 4 Antibody (YA4640) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • NADPH Oxidase 4 Antibody (YA4640) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • NADPH Oxidase 4 Antibody (YA4640) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using NADPH Oxidase 4 Antibody (YA4640). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80762A, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • NADPH Oxidase 4 Antibody (YA4640) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P80762A) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P80762A) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NADPH Oxidase 4 Antibody (YA4640) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P80762A) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P80762A) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NADPH Oxidase 4 Antibody (YA4640) Product Image WB
  • NADPH Oxidase 4 Antibody (YA4640) Product Image IHC-P
  • NADPH Oxidase 4 Antibody (YA4640) Product Image IHC-P
  • NADPH Oxidase 4 Antibody (YA4640) Product Image IHC-P
  • NADPH Oxidase 4 Antibody (YA4640) Product Image IHC-P
  • NADPH Oxidase 4 Antibody (YA4640) Product Image IHC-P
  • NADPH Oxidase 4 Antibody (YA4640) Product Image IHC-P
  • NADPH Oxidase 4 Antibody (YA4640) Product Image ICC
  • NADPH Oxidase 4 Antibody (YA4640) Product Image ICC