HDAC8 Antibody (YA7697)

Cat. No.: HY-P88012: Data Sheet User Guide for Antibodies
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HDAC8 Antibody (YA7697) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HDAC8.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

HDAC8 Antibody (YA7697) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HDAC8.

Host

Rabbit

Clonality

Recombinant

Molecular Weight

Predicted band size: 42 kDa;

Species Reactivity

Human

SwissProt ID

Q9BY41

Immunogen

A synthesized peptide derived from human HDAC8

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
FC FC: Flow Cytometry	1:50-100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:10748112, PubMed:10922473, PubMed:10926844, PubMed:14701748, PubMed:28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:10748112, PubMed:10922473, PubMed:10926844, PubMed:14701748). Histone deacetylases act via the formation of large multiprotein complexes (PubMed:10748112, PubMed:10922473, PubMed:10926844, PubMed:14701748). Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin (PubMed:22885700). May play a role in smooth muscle cell contractility (PubMed:15772115). In addition to protein deacetylase activity, also has protein-lysine deacylase activity: acts as a protein decrotonylase by mediating decrotonylation ((2E)-butenoyl) of histones (PubMed:28497810)

Subcellular Localization：Nucleus,Chromosome,Cytoplasm

Expression：
Tissue_Specificity: Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney

Isoforms & Post-Translational Modification：Q9BY41 has 6 isomers: Q9BY41-1: 41758 Da (predicted); Q9BY41-4: 31935 Da (predicted); Q9BY41-5: 28353 Da (predicted); Q9BY41-6: 15685 Da (predicted); Q9BY41-7: 17439 Da (predicted); Q9BY41-8: 16050 Da (predicted).
Phosphorylated by PKA on serine 39

Subunit：Interacts with PEPB2-MYH11, a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11 produced by the inversion Inv(16)(p13q22), a translocation associated with acute myeloid leukemia of M4EO subtype (PubMed:12509458)

Documentation

User Guide for Antibodies (1077 KB)