Doublecortin Antibody (YA5769)
(Synonyms: DCX; DBCN; LISX; Neuronal migration protein doublecortin; Doublin; Lissencephalin-X; Lis-X)
Based on 1 Customer Validation
Doublecortin Antibody (YA5769) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Doublecortin.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IP, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:2000-1:10000 | 1:5000-1:20000 | 1:50-1:200 |
Product Details
Doublecortin Antibody (YA5769) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Doublecortin.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 41 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 41 kDa
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Doublecortin is a Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration
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Subcellular Localization
Cytoplasm; Cell projection, neuron projection
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Expression
Tissue_specificity:It is highly expressed in fetal brain neurons (most cells in the cortical plate, intermediate zone, and ventricular zone) , but not in other fetal tissues. In humans, it is highly expressed in the frontal lobe of the brain, but very low in other brain regions, and is not detected in the heart, placenta, lungs, liver, skeletal muscle, kidneys, and pancreas. -
Isoforms & Post-Translational Modification
O43602 has 2 isomers: O43602-1: 40574 Da (predicted); O43602-2: 40044 Da (predicted).
Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules (By similarity). Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21 (By similarity);Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells -
Subunit
Interacts with tubulin (PubMed:27292316). Interacts with USP9X (PubMed:24607389)
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SwissProt ID
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Synonyms
DCX; DBCN; LISX; Neuronal migration protein doublecortin; Doublin; Lissencephalin-X; Lis-X
Documentation
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Data Sheet (234 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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User Guide for Antibodies (1077 KB)
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