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Cas9 SA Antibody (YA1683)

(Synonyms: Cas9; associated endonuclease Cas9/Csn1; Cas9/Csn1; csn1; SpyCas9)
Customer Review

Based on 1 Customer Validation

Cas9 SA Antibody (YA1683) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cas9 SA.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC

  • Reactivity :

    Staphylococcus aureus

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cas9 SA Antibody (YA1683)
  • Cas9 SA Antibody (YA1683) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:30 1:50-1:100

Product Details

Description

Cas9 SA Antibody (YA1683) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cas9 SA.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Staphylococcus aureus
  • Observed Molecular Weight
    Observed band size: 124 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 124 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from Cas9 aa1-1053.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104059

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cas9 SA Antibody (YA1683)
    Western blot analysis of extracts from HEK293 lysates transfected with Cas9 (lane 2(20μg), HEK293 lysates transfected with Cas9 (lane 3(40μg), using Cas9 SA Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Cas9 SA is a CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity

  • Subunit

    Monomer. Binds crRNA and tracrRNA

  • SwissProt ID

    J7RUA5

  • Synonyms

    Cas9; associated endonuclease Cas9/Csn1; Cas9/Csn1; csn1; SpyCas9

  • Research Field

    Microbiology

Cas9 SA Antibody (YA1683) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 lysates transfected with Cas9 (lane 2(20μg), HEK293 lysates transfected with Cas9 (lane 3(40μg), using Cas9 SA Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Cas9 SA Antibody (YA1683) Product Image
    1/16
  • Experimental Validation Results for Cas9 SA Antibody (YA1683)
  • Cas9 SA Antibody (YA1683) Product Image
  • Experimental Validation Results for Cas9 SA Antibody (YA1683)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 lysates transfected with Cas9 (lane 2(20μg), HEK293 lysates transfected with Cas9 (lane 3(40μg), using Cas9 SA Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cas9 SA Antibody (YA1683)
  • Cas9 SA Antibody (YA1683) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 lysates transfected with Cas9 (lane 2(20μg), HEK293 lysates transfected with Cas9 (lane 3(40μg), using Cas9 SA Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Cas9 SA Antibody (YA1683) Product Image WB