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  5. Cas9 SA Antibody (YA1683)

Cas9 SA Antibody (YA1683)

Cat. No.: HY-P81938
COA
SDS
User Guide for Antibodies Technical Support

Cas9 SA Antibody (YA1683) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cas9 SA.

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10 μL 해외재고보유
50 μL 해외재고보유
100 μL 해외재고보유
250 μL   견적 받기  
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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제품 설명

Cas9 SA Antibody (YA1683) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cas9 SA.
Host

Rabbit
Clonality

Recombinant,Monoclonal
분자량
Predicted band size: 124 kDa;
Observed band size: 124 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Staphylococcus aureus
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from Cas9 aa1-1053.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
IP
IP: Immunoprecipitation
1:30
FC
FC: Flow Cytometry
1:50-1:100
Sensitivity Endogenous Purity Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from HEK293 lysates transfected with Cas9 (lane 2(20μg), HEK293 lysates transfected with Cas9 (lane 3(40μg), using Cas9 SA Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background
Function：CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity
Subunit：Monomer. Binds crRNA and tracrRNA
RRID
Database

SwissProt: J7RUA5 Staphylococcus

Research Field

Microbiology
Synonyms
Cas9; associated endonuclease Cas9/Csn1; Cas9/Csn1; csn1; SpyCas9
각종 서류
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Cas9 SA Antibody (YA1683) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
Cas9 SA Antibody (YA1683)
Cat. No.:
HY-P81938
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SAFETY DATA SHEET (SDS)

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