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ALAS1 Antibody (YA3021)

(Synonyms: ALAS1; ALAS-H; 5-aminolevulinic acid synthase 1; Delta-ALA synthase 1; ALAS3; ALASH)
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Based on 1 Customer Validation

ALAS1 Antibody (YA3021) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALAS1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ALAS1 Antibody (YA3021)
  • ALAS1 Antibody (YA3021) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

ALAS1 Antibody (YA3021) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALAS1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 71 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 71 kDa
Species Reactivity Database

Entrez Gene: 211 Human

SwissProt: P13196 Human

Immunogen

A synthesized peptide derived from human Alas1 aa600-640.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105390

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ALAS1 Antibody (YA3021)
    Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), JAR (lane 2, 15 μg), K562 (lane 3, 15 μg), Hela (lane 4, 15 μg), Raji (lane 5, 15 μg), and Jurkat (lane 6, 15 μg) using ALAS1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin , HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    ALAS1 catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products

  • Subcellular Localization

    Mitochondrion inner membrane; Peripheral membrane protein

  • Expression


    Induction:Up-regulated by bile acids; chenodeoxycholic acid, ursodeoxycholic acid and lithocholic acid and by the NR1H4/FXR-specific agonist GW4064

  • Isoforms & Post-Translational Modification

    P13196 has 2 isomers: P13196-1: 70581 Da (predicted); P13196-2: 12961 Da (predicted).
    In normoxia, is hydroxylated at Pro-576, promoting interaction with VHL, initiating ubiquitination and subsequent degradation via the proteasome;Ubiquitinated; in normoxia following hydroxylation and interaction with VHL, leading to its subsequent degradation via the proteasome

  • Subunit

    Homodimer (By similarity). Interacts (hydroxylated form) with VHL (PubMed:16234850)

  • SwissProt ID

    P13196

  • Gene ID
    211 [NCBI]
  • Synonyms

    ALAS1; ALAS-H; 5-aminolevulinic acid synthase 1; Delta-ALA synthase 1; ALAS3; ALASH

  • Research Field

    Cardiovascular

ALAS1 Antibody (YA3021) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), JAR (lane 2, 15 μg), K562 (lane 3, 15 μg), Hela (lane 4, 15 μg), Raji (lane 5, 15 μg), and Jurkat (lane 6, 15 μg) using ALAS1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin , HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ALAS1 Antibody (YA3021) Product Image
    1/16
  • Experimental Validation Results for ALAS1 Antibody (YA3021)
  • ALAS1 Antibody (YA3021) Product Image
  • Experimental Validation Results for ALAS1 Antibody (YA3021)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), JAR (lane 2, 15 μg), K562 (lane 3, 15 μg), Hela (lane 4, 15 μg), Raji (lane 5, 15 μg), and Jurkat (lane 6, 15 μg) using ALAS1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin , HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ALAS1 Antibody (YA3021)
  • ALAS1 Antibody (YA3021) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), JAR (lane 2, 15 μg), K562 (lane 3, 15 μg), Hela (lane 4, 15 μg), Raji (lane 5, 15 μg), and Jurkat (lane 6, 15 μg) using ALAS1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin , HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ALAS1 Antibody (YA3021) Product Image WB