ALAS1 Antibody (YA3021)

Cat. No.: HY-P83276: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

ALAS1 Antibody (YA3021) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALAS1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

ALAS1 Antibody (YA3021) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

ALAS1 Antibody (YA3021) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALAS1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 71 kDa;

Observed band size: 71 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P13196

Gene ID

211 [NCBI]

Immunogen

A synthesized peptide derived from human Alas1 aa600-640.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), JAR (lane 2, 15 μg), K562 (lane 3, 15 μg), Hela (lane 4, 15 μg), Raji (lane 5, 15 μg), and Jurkat (lane 6, 15 μg) using ALAS1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin , HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products

Subcellular Localization：Mitochondrion inner membrane; Peripheral membrane protein

Expression：
Induction:Up-regulated by bile acids; chenodeoxycholic acid, ursodeoxycholic acid and lithocholic acid and by the NR1H4/FXR-specific agonist GW4064

Isoforms & Post-Translational Modification：P13196 has 2 isomers: P13196-1: 70581 Da (predicted); P13196-2: 12961 Da (predicted).
In normoxia, is hydroxylated at Pro-576, promoting interaction with VHL, initiating ubiquitination and subsequent degradation via the proteasome;Ubiquitinated; in normoxia following hydroxylation and interaction with VHL, leading to its subsequent degradation via the proteasome

Subunit：Homodimer (By similarity). Interacts (hydroxylated form) with VHL (PubMed:16234850)

RRID

AB_3105390

Database

Entrez Gene: 211 Human

SwissProt: P13196 Human

OMIM: 125290 Human

Research Field

Cardiovascular

Synonyms

ALAS1; ALAS-H; 5-aminolevulinic acid synthase 1; Delta-ALA synthase 1; ALAS3; ALASH

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

ALAS1 Antibody (YA3021) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Country or Region *

Email Address *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

ALAS1 Antibody (YA3021)

ALAS1 Antibody (YA3021) Featured Recommendations:

Product Detail

Verification Image

Background

Documentation

ALAS1 Antibody (YA3021) Related Classifications

Your Recently Viewed Products:

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report