Copy Link Print Information

CD58 Antibody (YA1305)

(Synonyms: Ag3, LFA3, FLJ23181, CD58)
Customer Review

Based on 1 Customer Validation

CD58 Antibody (YA1305) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD58.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for CD58 Antibody (YA1305)
  • Experimental Validation Results for CD58 Antibody (YA1305)
  • Experimental Validation Results for CD58 Antibody (YA1305)
  • CD58 Antibody (YA1305) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000 1:1000

Product Details

Description

CD58 Antibody (YA1305) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD58.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 55-70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 28 kDa
Species Reactivity Database

Entrez Gene: 965 Human

SwissProt: P19256 Human

Immunogen

Recombinant protein within human CD58 aa 29-215 (Extracellular).

Sensitivity

Endogenous

Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD58 Antibody (YA1305)
    Western blot analysis of extracts from Hela (lane2, 20μg), Raji (lane3, 20μg) and Jurkat (lane4, 20μg) using CD58 Antibody (HY-P81560). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD58 Antibody (YA1305)
    Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2), HeLa (lane 3), MCF-7 (lane 4), 293T (lane 5), HT-1080 (lane 6), and A549 (lane 7) using CD58 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for CD58 Antibody (YA1305)
    Flow cytometric analysis of 1X106 Raji cells labeling CD58 Antibody (HY-P81560, red). Cells were stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    CD58 is a Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Isoforms & Post-Translational Modification

    P19256 has 3 isomers: P19256-1: 28147 Da (predicted); P19256-2: 26661 Da (predicted); P19256-3: 27960 Da (predicted).

  • Subunit

    Interacts with CD2 (PubMed:10380930, PubMed:3309127). Interacts with CMTM6 (PubMed:28813417)

  • SwissProt ID

    P19256

  • Gene ID
    965 [NCBI]
  • Synonyms

    Ag3, LFA3, FLJ23181, CD58

  • Research Field

    Immunology

CD58 Antibody (YA1305) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), Raji (lane3, 20μg) and Jurkat (lane4, 20μg) using CD58 Antibody (HY-P81560). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2), HeLa (lane 3), MCF-7 (lane 4), 293T (lane 5), HT-1080 (lane 6), and A549 (lane 7) using CD58 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Raji cells labeling CD58 Antibody (HY-P81560, red). Cells were stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD58 Antibody (YA1305) Product Image
    1/16
  • Experimental Validation Results for CD58 Antibody (YA1305)
  • Experimental Validation Results for CD58 Antibody (YA1305)
  • Experimental Validation Results for CD58 Antibody (YA1305)
  • CD58 Antibody (YA1305) Product Image
  • Experimental Validation Results for CD58 Antibody (YA1305)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), Raji (lane3, 20μg) and Jurkat (lane4, 20μg) using CD58 Antibody (HY-P81560). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD58 Antibody (YA1305)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2), HeLa (lane 3), MCF-7 (lane 4), 293T (lane 5), HT-1080 (lane 6), and A549 (lane 7) using CD58 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for CD58 Antibody (YA1305)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Raji cells labeling CD58 Antibody (HY-P81560, red). Cells were stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CD58 Antibody (YA1305)
  • Experimental Validation Results for CD58 Antibody (YA1305)
  • Experimental Validation Results for CD58 Antibody (YA1305)
  • CD58 Antibody (YA1305) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2, 20μg), Raji (lane3, 20μg) and Jurkat (lane4, 20μg) using CD58 Antibody (HY-P81560). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CD58 Antibody (YA1305) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2), HeLa (lane 3), MCF-7 (lane 4), 293T (lane 5), HT-1080 (lane 6), and A549 (lane 7) using CD58 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • CD58 Antibody (YA1305) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 Raji cells labeling CD58 Antibody (HY-P81560, red). Cells were stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD58 Antibody (YA1305) Product Image WB
  • CD58 Antibody (YA1305) Product Image WB
  • CD58 Antibody (YA1305) Product Image FC