CD58 Antibody (YA1305)

Cat. No.: HY-P81560: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

CD58 Antibody (YA1305) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD58.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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CD58 Antibody (YA1305) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation
References

Description

CD58 Antibody (YA1305) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD58.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 28 kDa;

Observed band size: 55-70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P19256

Gene ID

965 [NCBI]

Immunogen

Recombinant protein within human CD58 aa 29-215 (Extracellular).

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
FC FC: Flow Cytometry	1:1000

Sensitivity	Endogenous	Purity	Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2), HeLa (lane 3), MCF-7 (lane 4), 293T (lane 5), HT-1080 (lane 6), and A549 (lane 7) using CD58 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot^® Turbo^™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

Function：Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells

Subcellular Localization：Cell membrane; Single-pass type I membrane protein

Isoforms & Post-Translational Modification：P19256 has 3 isomers: P19256-1: 28147 Da (predicted); P19256-2: 26661 Da (predicted); P19256-3: 27960 Da (predicted).

Subunit：Interacts with CD2 (PubMed:10380930, PubMed:3309127). Interacts with CMTM6 (PubMed:28813417)

Database

Entrez Gene: 965 Human

SwissProt: P19256 Human

OMIM: 153420 Human

Research Field

Immunology

Synonyms

Ag3, LFA3, FLJ23181, CD58

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/pc-3-cell.html [Content Brief]

CD58 Antibody (YA1305) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CD58 Antibody (YA1305)

CD58 Antibody (YA1305) Featured Recommendations:

Product Detail

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Background

Documentation

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CD58 Antibody (YA1305) Related Classifications

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