Histone H1.3 Antibody (YA3006)

Art. -Nr.: HY-P83261: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Histone H1.3 Antibody (YA3006) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Histone H1.3.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Größe	Verfügbarkeit	Menge
Kostenlose Probe	Apply now
10 μL	Auf Lager	Estimated Time of Arrival: December 31
50 μL	Auf Lager	Estimated Time of Arrival: December 31
100 μL	Auf Lager	Estimated Time of Arrival: December 31
250 μL	Angebot einholen

or Massenanfrage

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Histone H1.3 Antibody (YA3006) Spezialitätsempfehlungen:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Beschreibung

Beschreibung

Histone H1.3 Antibody (YA3006) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Histone H1.3.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molekulargewicht

Predicted band size: 22 kDa;

Observed band size: 30 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P16402

Gene ID

3008 [NCBI]

Immunogen

A synthetic peptide of human Histone H13 aa5-17.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Reinheit	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from Hela(lane 2(20ug)，C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Histone H1.3 Antibody (HY-P83261) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling Histone H1.3 Antibody (HY-P83261) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H1.3 Antibody (HY-P83261) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of Hela cells labeling Histone H1.3 Antibody (HY-P83261) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H1.3 Antibody (HY-P83261) at 1/150 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Also acts as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity)

Subcellular Localization：Nucleus; Chromosome

RRID

AB_3105375

Database

Entrez Gene: 3008 Human ; 50709 Mouse ;

SwissProt: P10412 Human ; P16402 Human ; P43274 Mouse ;

OMIM: 617537 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

Histone H1c; Histone H1s-2

Dokumentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Histone H1.3 Antibody (YA3006) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Ihre kürzlich angesehenen Produkte:

Online-Anfrage

Your information is safe with us. * Required Fields.

Please select a local distributor ナミキ商事株式会社コスモ・バイオ株式会社重松貿易株式会社
Produktname			Requested Quantity *
Name des Antragstellers *			Anrede
E-Mail-Addresse *			Telefonnummer *
Department			Name der Organisation *
City			Bundesland
Country or Region *
Bemerkungen

Massenanfrage

Inquiry Information

Produktname:: Histone H1.3 Antibody (YA3006)
Art. -Nr.:: HY-P83261
Menge:
MCE Japan Authorized Agent:

1/16

MedchemExpress Validation
Western blot analysis of extracts from Hela(lane 2(20ug)，C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Histone H1.3 Antibody (HY-P83261) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
1/16

MedChemExpress Validation
Immunocytochemistry analysis of Hela cells labeling Histone H1.3 Antibody (HY-P83261) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H1.3 Antibody (HY-P83261) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
1/16

MedChemExpress Validation
Immunocytochemistry analysis of Hela cells labeling Histone H1.3 Antibody (HY-P83261) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H1.3 Antibody (HY-P83261) at 1/150 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
1/16

Produktname			Lot #
Name des Antragstellers *			Country or Region *
E-Mail-Addresse *
Telefonnummer *			Name der Organisation *
Bemerkungen

MedChemExpress: Contact Us; About Us; Headquarters; Distributors; Careers

Kunden Service: Technical Support; Dienstleistungen; Ordering Information; Easy Return; Versandkosten & Richtlinien; Intellectual Property Promise

Ressourcen: Richtlinien für Kostenlose Proben; Resources; Molaritätsrechner; Verdünnungsrechner; Terms & Conditions; Reconstitution Calculator; Specific Activity Calculator; DNA/RNA sequence conversion tools

Abonnieren Sie unseren E-Newsletter

Name
Email *

	Sorry, but the email address you supplied was invalid.

Produkte sind chemische Reagenzien, die nur für Forschungszwecke bestimmt sind und nicht für den menschlichen Gebrauch. Wir verkaufen nicht an Patienten.

Join with us

Histone H1.3 Antibody (YA3006)

Histone H1.3 Antibody (YA3006) Spezialitätsempfehlungen:

Product Detail

Verification Image

Background

Beschreibung

Histone H1.3 Antibody (YA3006) Related Classifications

Ihre kürzlich angesehenen Produkte:

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report