Phospho-Cofilin (Ser3) Antibody (YA9871)
(Synonyms: 18 kDa phosphoprotein; CFL 1; CFL; CFL1; COF1_HUMAN; Cofilin 1; Cofilin 1 non muscle; Cofilin; Cofilin non muscle isoform; Cofilin-1; epididymis secretory protein Li 15; HEL-S-15; non-muscle isoform; p18)
Based on 1 Customer Validation
Phospho-Cofilin (Ser3) Antibody (YA9871) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospho-Cofilin (Ser3).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Rat, Monkey
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:2000 |
Product Details
Phospho-Cofilin (Ser3) Antibody (YA9871) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospho-Cofilin (Ser3).
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Host Rabbit
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Species ReactivityHuman, Rat, Monkey
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Observed Molecular WeightObserved band size: 19 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 19 kDa
Synthetic phospho-peptide corresponding to residues surrounding Ser3 of human Cofilin aa 1-50.
Endogenous
affinity purified
Non-conjugated
Phosphorylated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from C6 (lane 2, 40ug) using Phospho-Cofilin (Ser3) Antibody (HY-P810609). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
Background
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Function
Cofilin binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration (By similarity)[1][2][3][4][5].
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Subcellular Localization
Nucleus matrix; Cytoplasm, cytoskeleton; Cell projection, ruffle membrane; Cell projection, lamellipodium membrane; Cell projection, lamellipodium; Cell projection, growth cone; Cell projection, axon
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Expression
Tissue_Specificity: Widely distributed in various tissues.
Induction: Up-regulated in response to enterovirus 71 (EV71) infection (at protein level). -
Isoforms & Post-Translational Modification
Phospho-Cofilin has an amino acid length of 166, molecular weight is 18502 Da.
Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity). Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2. -
Subunit
Can bind G- and F-actin in a 1:1 ratio of cofilin to actin.
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SwissProt ID
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Synonyms
18 kDa phosphoprotein; CFL 1; CFL; CFL1; COF1_HUMAN; Cofilin 1; Cofilin 1 non muscle; Cofilin; Cofilin non muscle isoform; Cofilin-1; epididymis secretory protein Li 15; HEL-S-15; non-muscle isoform; p18
Documentation
References
[1]. Yeoh S, et al. Determining the differences in actin binding by human ADF and cofilin. J Mol Biol. 2002 Jan 25;315(4):911-25. [Content Brief]
[2]. Hsiao BY, et al. Human Costars Family Protein ABRACL Modulates Actin Dynamics and Cell Migration and Associates with Tumorigenic Growth. Int J Mol Sci. 2021 Feb 18;22(4):. [Content Brief]
[3]. Gohla A, et al. Chronophin, a novel HAD-type serine protein phosphatase, regulates cofilin-dependent actin dynamics. Nat Cell Biol. 2005 Jan;7(1):21-9. [Content Brief]
[4]. Bai SW, et al. Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration. BMC Biol. 2011 Aug 11;9:54. [Content Brief]
[5]. Borroni EM, et al. β-arrestin-dependent activation of the cofilin pathway is required for the scavenging activity of the atypical chemokine receptor D6. Sci Signal. 2013 Apr 30;6(273):ra30.1-11, S1-3. [Content Brief]
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