Copy Link Print Information

FSP27/CIDEC Antibody

(Synonyms: Cell Death Activator; Cell death activator CIDE-3; Cell death inducing DFFA like effector protein C; CIDE 3; CIDE C; CIDEC_HUMAN; FLJ20871; FPLD5; FSP27; CIDE-3)
1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

FSP27/CIDEC Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to FSP27/CIDEC.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ELISA, IHC-P, IHC-F, FC, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for FSP27/CIDEC Antibody
  • FSP27/CIDEC Antibody Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) FSP27/CIDEC Antibody

More

Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
FC Info
FC: Flow Cytometry
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-2000 1:5000-10000 1:100-500 1:100-500 1ug:Test 1:100-500

Product Details

Description

FSP27/CIDEC Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to FSP27/CIDEC.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat Predicted Reactivity: Pig,
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 27 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 27 kDa
Species Reactivity Database
Immunogen

KLH conjugated synthetic peptide derived from human CIDEC: 101-200/238

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103048

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FSP27/CIDEC Antibody
    Western blot analysis of extracts from HepG2 using CIDEC/FSP27 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    FSP27/CIDEC is a Lipid transferase specifically expressed in white adipose tissue, which promotes unilocular lipid droplet formation by mediating lipid droplet fusion. Lipid droplet fusion promotes their enlargement, restricting lipolysis and favoring lipid storage. Localizes on the lipid droplet surface, at focal contact sites between lipid droplets, and mediates atypical lipid droplet fusion by undergoing liquid-liquid phase separation (LLPS) and promoting directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair. Its role in neutral lipid transfer and lipid droplet enlargement is activated by the interaction with PLIN1. May also act as a CEBPB coactivator in the white adipose tissue to control the expression of a subset of CEBPB downstream target genes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH. When overexpressed in preadipocytes, induces apoptosis or increases cell susceptibility to apoptosis induced by serum deprivation or TGFB treatment[1][2][3][4][5][6].

  • Subcellular Localization

    Lipid droplet; Endoplasmic reticulum; Nucleus

  • Expression


    Tissue_specificity:It is mainly expressed in adipose tissue, small intestine, heart, colon and stomach, with lower expression levels in brain, kidney and liver.

  • Isoforms & Post-Translational Modification

    Q96AQ7 has 4 isomers: Q96AQ7-1: 26754 Da (predicted); Q96AQ7-2: 18439 Da (predicted); Q96AQ7-3: 14013 Da (predicted); Q96AQ7-4: 27923 Da (predicted).
    Ubiquitinated and targeted to proteasomal degradation, resulting in a short half-life (about 15 minutes in 3T3-L1 cells). Protein stability depends on triaclyglycerol synthesis, fatty acid availability and lipid droplet formation

  • Subunit

    Homodimer (By similarity). Homooligomer; undergoes liquid-liquid phase separation (LLPS) via its N-terminus, facilitating lipid droplet fusion, occurs at the lipid droplet contact sites (By similarity). Interacts with CIDEA (PubMed:19843876). Interacts with PLIN1 (PubMed:23399566). Interacts with NFAT5; this interaction is direct and retains NFAT5 in the cytoplasm (By similarity). Interacts with CEBPB (By similarity). Interacts with isoform CLSTN3beta of CLSTN3; inhibiting the lipid transferase activity of CIDEC (By similarity)

  • SwissProt ID

    Q96AQ7

  • Gene ID
    63924 [NCBI]
  • Synonyms

    Cell Death Activator; Cell death activator CIDE-3; Cell death inducing DFFA like effector protein C; CIDE 3; CIDE C; CIDEC_HUMAN; FLJ20871; FPLD5; FSP27; CIDE-3

  • Research Field

    Cell Biology

References

[1]. Keller P, et al. Fat-specific protein 27 regulates storage of triacylglycerol. J Biol Chem. 2008 May 23;283(21):14355-65. [Content Brief]

[2]. Liu K, et al. Functional analysis of FSP27 protein regions for lipid droplet localization, caspase-dependent apoptosis, and dimerization with CIDEA. Am J Physiol Endocrinol Metab. 2009 Dec;297(6):E1395-413. [Content Brief]

[3]. Rubio-Cabezas O, et al. Partial lipodystrophy and insulin resistant diabetes in a patient with a homozygous nonsense mutation in CIDEC. EMBO Mol Med. 2009 Aug;1(5):280-7. [Content Brief]

[4]. Grahn TH, et al. FSP27 and PLIN1 interaction promotes the formation of large lipid droplets in human adipocytes. Biochem Biophys Res Commun. 2013 Mar 8;432(2):296-301. [Content Brief]

[5]. Wang J, et al. Polybasic RKKR motif in the linker region of lipid droplet (LD)-associated protein CIDEC inhibits LD fusion activity by interacting with acidic phospholipids. J Biol Chem. 2018 Dec 14;293(50):19330-19343. [Content Brief]

[6]. Liang L, et al. Molecular cloning and characterization of CIDE-3, a novel member of the cell-death-inducing DNA-fragmentation-factor (DFF45)-like effector family. Biochem J. 2003 Feb 15;370(Pt 1):195-203. [Content Brief]

View More

FSP27/CIDEC Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HepG2 using CIDEC/FSP27 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • FSP27/CIDEC Antibody Product Image
    1/16
  • Experimental Validation Results for FSP27/CIDEC Antibody
  • FSP27/CIDEC Antibody Product Image
  • Experimental Validation Results for FSP27/CIDEC Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HepG2 using CIDEC/FSP27 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for FSP27/CIDEC Antibody
  • FSP27/CIDEC Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HepG2 using CIDEC/FSP27 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • FSP27/CIDEC Antibody Product Image WB