GATA4 Antibody (YA1568)

Cat. No.: HY-P81823: Ficha de datos COA
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GATA4 Antibody (YA1568) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GATA4.

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10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
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GATA4 Antibody (YA1568) Recomendaciones destacadas:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Descripciòn
Referencias

Descripciòn

GATA4 Antibody (YA1568) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GATA4.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Peso molecular

Predicted band size: 45 kDa;

Observed band size: 54 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P43694

Gene ID

2626 [NCBI]

Immunogen

A synthesized peptide derived from human GATA4 aa5-48.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Pureza	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg) using GATA4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function (PubMed:24000169, PubMed:27984724, PubMed:35182466). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it down-regulates endocardial and endothelial gene expression (PubMed:27984724). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase (PubMed:15734735)

Subcellular Localization：Nucleus

Isoforms & Post-Translational Modification：P43694 has 2 isomers: P43694-1: 44565 Da (predicted); P43694-2: 44665 Da (predicted).
Methylation at Lys-300 attenuates transcriptional activity

Subunit：Interacts with ZNF260 (By similarity). Interacts with the homeobox domain of NKX2-5 through its C-terminal zinc finger. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts (via the second Zn finger) with NFATC4 (By similarity). Interacts with LMCD1 (By similarity). Forms a complex made of CDK9, CCNT1/cyclin-T1, EP300 and GATA4 that stimulates hypertrophy in cardiomyocytes. Interacts with NR5A1, ZFPM2 and TBX5. Interacts with TBX18. Interacts with GLYR1; the interaction is required for a synergistic activation of GATA4 target genes transcription (PubMed:35182466). Interacts with PHF7; the interaction promotes GATA4 binding to its transcription targets (By similarity)

RRID

AB_3103923

Database

Entrez Gene: 2626 Human ; 14463 Mouse ; 54254 Rat

SwissProt: P43694 Human ; Q08369 Mouse ; P46152 Rat

OMIM: 607941 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

GATA4; Transcription factor GATA-4; GATA-binding factor 4

Documentación

Select Batch:

Ficha de datos (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/a549-cell.html [Content Brief]

GATA4 Antibody (YA1568) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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GATA4 Antibody (YA1568)

GATA4 Antibody (YA1568) Recomendaciones destacadas:

Product Detail

Verification Image

Background

Descripciòn

Referencias

GATA4 Antibody (YA1568) Related Classifications

Productos vistos recientemente:

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