WDR77 Antibody (YA655)(PBS only)

Cat. No.: HY-P80931A: Data Sheet User Guide for Antibodies
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WDR77 Antibody (YA655) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to WDR77.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

WDR77 Antibody (YA655) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to WDR77.

Host

Mouse

Molecular Weight

Predicted band size: 37 kDa;

Observed band size: 42 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9BQA1

Gene ID

79084 [NCBI]

Immunogen

Synthetic peptide corresponding to Human WDR77.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones (PubMed:11756452). This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (PubMed:23071334)

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Tissue_specificity:It is highly expressed in the heart, skeletal muscle, spleen, testes, uterus, prostate, and thymus. In the testes, it is expressed in germ cells and interstitial cells, but not in peritubular muscle cells and Sertoli cells. It is expressed in prostate cancer, seminoma, and interstitial cell tumors.

Isoforms & Post-Translational Modification：Q9BQA1 has 2 isomers: Q9BQA1-1: 36724 Da (predicted); Q9BQA1-2: 29651 Da (predicted).

Subunit：Component of the methylosome complex composed of PRMT5, WDR77 and CLNS1A (PubMed:18984161, PubMed:21081503). Found in a complex composed of PRMT5, WDR77 and RIOK1 (PubMed:21081503). RIOK1 and CLNS1A bound directly to PRMT5 at the same binding site, in a mutually exclusive manner, which allows the recruitment of distinct methylation substrates, such as nucleolin/NCL and Sm proteins, respectively (PubMed:21081503). Found in a complex with the component of the methylosome, PRMT5, CLNS1A, WDR77, PRMT1 and ERH (PubMed:25284789). Directly interacts with PRMT5, as well as with several Sm proteins, including SNRPB and SNRPD2 and, more weakly, SNRPD3 and SNRPE (PubMed:11756452, PubMed:33376131). Forms a compact hetero-octamer with PRMT5, decorating the outer surface of a PRMT5 tetramer. Interacts with SUZ12 and histone H2A/H2AC20, but not with histones H2B, H3 nor H4 (PubMed:16712789). Interacts with CTDP1 and LSM11 (PubMed:12560496, PubMed:16087681). Interacts with APEX1, AR and NKX3-1 (PubMed:12972618, PubMed:19188445). Interacts with CHTOP (PubMed:25284789). Interacts with FAM47E. Interacts with TSC22D2 (PubMed:27337956)

Research Field

Signal Transduction

Synonyms

WD repeat domain 77; p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50

Documentation

User Guide for Antibodies (1077 KB)