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STING Antibody (YA048)

(Synonyms: ERIS, MITA, STING, TMEM173, STING1, Stimulator of interferon genes protein, hSTING, Endoplasmic reticulum interferon stimulator, Mediator of IRF3 activation, Transmembrane protein 173, hMITA)
Customer Review

Based on 1 Customer Validation

STING Antibody (YA048) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STING.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for STING Antibody (YA048)
  • Experimental Validation Results for STING Antibody (YA048)
  • Experimental Validation Results for STING Antibody (YA048)
  • STING Antibody (YA048) Product Image
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Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

STING Antibody (YA048) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STING.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database

Entrez Gene: 340061 Human

SwissProt: Q86WV6 Human

Immunogen

Synthetic peptide corresponding to Human TMEM173.AA range:220-379.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102448

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for STING Antibody (YA048)
    Western blot analysis of extracts from THP-1 (lane 2(20μg) , THP-1 (lane 3(40μg),using STING Antibody (HY-P80350). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for STING Antibody (YA048)
    Western blot analysis of extracts from THP-1(lane 2(20μg), THP-1 (lane 3(40μg),using STING Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for STING Antibody (YA048)
    Flow cytometric analysis of 1X10^6 THP-1 cells labeling STING Antibody (HY-P80350, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Stimulator of interferon genes (STING) is an integral ER-membrane protein that can be activated by 2'3'-cGAMP synthesized by cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS) upon binding of double-stranded DNA. It activates interferon (IFN) and inflammatory cytokine responses to defend against infection by microorganisms.

    STING is a key cytosolic receptor for small nucleotides and plays a key role in anticancer and antiviral immunity. STING signaling pathway is also a critical link between innate and adaptive immunity, and induces anti-tumor immune responses. STING agonists, such as endogenous cyclic dinucleotide (CDN) cyclic GMP-AMP (cGAMP)， have been used in diverse research for immunogenic tumor clearance, antiviral treatments and vaccine adjuvants.

  • Subcellular Localization

    Endoplasmic reticulum membrane; Multi-pass membrane protein; Cytoplasm, perinuclear region; Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein; Golgi apparatus membrane; Multi-pass membrane protein; Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein; Mitochondrion outer membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Widely expressed (PubMed:18724357, PubMed:18818105) . Expressed in skin endothelial cells, type II alveolar cells, bronchial epithelial cells, and alveolar macrophages (PubMed:25029335) .

  • Subunit

    Homodimer; forms a homodimer in absence of cyclic nucleotide (c-di-GMP or cGAMP); 'Lys-63'-linked ubiquitination at Lys-150 is required for homodimerization (PubMed:19285439, PubMed:22579474, PubMed:22705373, PubMed:22728658, PubMed:22728659, PubMed:22728660, PubMed:30842659). Homotetramer; in presence of cyclic nucleotide (c-di-GMP or cGAMP), forms tetramers and higher-order oligomers through side-by-side packing (PubMed:30842653, PubMed:35388221). Interacts (when phosphorylated) with IRF3; following activation and phosphorylation on the pLxIS motif by TBK1, recruits IRF3 (PubMed:22394562, PubMed:25636800, PubMed:27302953, PubMed:28331227). Interacts with RIGI, MAVS and SSR2 (PubMed:18724357, PubMed:18818105). Interacts with RNF5 and TRIM56 (PubMed:19285439, PubMed:21074459). Interacts with TBK1; when homodimer, leading to subsequent production of IFN-beta (PubMed:19416887). Interacts with IFIT1 and IFIT2 (PubMed:19416887). Interacts with TRIM29; this interaction induces STING1 ubiquitination and subsequent degradation (PubMed:29038422). Associates with the MHC-II complex (By similarity). Interacts with STEEP1; interaction takes place upon cGAMP-activation and STING1 phosphorylation by MAP3K7/TAK1 and promotes STING1 translocation to COPII vesicles (PubMed:32690950, PubMed:37832545). Interacts with SEC24A, SEC24B, and SEC24C; promoting translocation to COPII vesicles (PubMed:30842662, PubMed:32690950). Interacts (when ubiquitinated) with SQSTM1; leading to relocalization to autophagosomes (By similarity). Interacts with SURF4 (PubMed:29251827). Interacts with HNRNPA2B1 (PubMed:31320558). Interacts with ZDHHC1; ZDHHC1 constitutively interacts with STING1 and in presence of DNA viruses activates it by promoting its cGAMP-induced oligomerization and the recruitment of downstream signaling components (PubMed:25299331). Interacts with ZDHHC11; in presence of DNA viruses promotes the recruitment of IRF3 to STING1 (PubMed:28331227). Interacts with TOMM70 (PubMed:20628368). Interacts with isoform IFI16-beta of IFI16 (PubMed:30104205). Interacts with TAB1; promoting recruitment of TAB1 to the endoplasmic reticulum membrane and subsequent activation of MAP3K7/TAK1 (PubMed:37832545). Interacts (via transmembrane domain) with TMEM203 (By similarity). Interacts with DDX41 (PubMed:25704810). Interacts with TMEM120A (via C-terminal domain); regulates the trafficking of STING1 from the ER to the ER-Golgi intermediate compartment to elicit antiviral effects (PubMed:35013224)

  • SwissProt ID

    Q86WV6

  • Gene ID
    340061 [NCBI]
  • Synonyms

    ERIS, MITA, STING, TMEM173, STING1, Stimulator of interferon genes protein, hSTING, Endoplasmic reticulum interferon stimulator, Mediator of IRF3 activation, Transmembrane protein 173, hMITA

  • Research Field

    Immunology

STING Antibody (YA048) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from THP-1 (lane 2(20μg) , THP-1 (lane 3(40μg),using STING Antibody (HY-P80350). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from THP-1(lane 2(20μg), THP-1 (lane 3(40μg),using STING Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 THP-1 cells labeling STING Antibody (HY-P80350, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • STING Antibody (YA048) Product Image
    1/16
  • Experimental Validation Results for STING Antibody (YA048)
  • Experimental Validation Results for STING Antibody (YA048)
  • Experimental Validation Results for STING Antibody (YA048)
  • STING Antibody (YA048) Product Image
  • Experimental Validation Results for STING Antibody (YA048)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from THP-1 (lane 2(20μg) , THP-1 (lane 3(40μg),using STING Antibody (HY-P80350). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for STING Antibody (YA048)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from THP-1(lane 2(20μg), THP-1 (lane 3(40μg),using STING Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for STING Antibody (YA048)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 THP-1 cells labeling STING Antibody (HY-P80350, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for STING Antibody (YA048)
  • Experimental Validation Results for STING Antibody (YA048)
  • Experimental Validation Results for STING Antibody (YA048)
  • STING Antibody (YA048) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from THP-1 (lane 2(20μg) , THP-1 (lane 3(40μg),using STING Antibody (HY-P80350). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • STING Antibody (YA048) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from THP-1(lane 2(20μg), THP-1 (lane 3(40μg),using STING Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • STING Antibody (YA048) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 THP-1 cells labeling STING Antibody (HY-P80350, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • STING Antibody (YA048) Product Image WB
  • STING Antibody (YA048) Product Image WB
  • STING Antibody (YA048) Product Image FC