Thioredoxin Antibody (YA035)

Cat. No.: HY-P80912: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Thioredoxin Antibody (YA035) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thioredoxin.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Thioredoxin Antibody (YA035) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Thioredoxin Antibody (YA035) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thioredoxin.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 12 kDa;

Observed band size: 12 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P10599

Gene ID

7295 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Thioredoxin/TRX.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:10000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:500
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Jurkat(lane 2(20μg) and Jurkat(lane 3(40μg), Hela(lane 4(20μg) ) and Hela(lane 5(40μg) ) using Thioredoxin (HY-P80912) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80912, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), U-87MG (lane 2, 15 μg), SW480 (lane 3, 15 μg), and HT-1080 (lane 4, 15 μg) using Thioredoxin / TRX Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (PubMed:17182577, PubMed:19032234, PubMed:2176490). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity (PubMed:16408020, PubMed:17606900). Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (PubMed:11118054, PubMed:9108029); ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55)

Subcellular Localization：Nucleus; Cytoplasm; Secreted

Expression：
Induction:Up-regulated by ionizing radiation

Isoforms & Post-Translational Modification：P10599 has 2 isomers: P10599-1: 11737 Da (predicted); P10599-2: 9452 Da (predicted).
In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins;In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation

Subunit：Homodimer; disulfide-linked (PubMed:17260951, PubMed:9369469). Interacts with TXNIP through the redox-active site (PubMed:17260951). Interacts with MAP3K5 and CASP3 (PubMed:15246877). In case of infection, interacts with S.typhimurium protein slrP (PubMed:19690162). Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA-binding activity in a redox-dependent manner (PubMed:9108029)

RRID

AB_3102749

Database

Entrez Gene: 7295 Human

SwissProt: P10599 Human

OMIM: 187700 Human

Research Field

Tags & Cell Markers

Synonyms

TXN; ADF; ATL-derived factor; SASP; Thioredoxin; TRX1; Thioredoxin delta 3; TRX; TXN delta 3; TRDX

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Thioredoxin Antibody (YA035) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Thioredoxin Antibody (YA035)

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Background

Documentation

Thioredoxin Antibody (YA035) Related Classifications

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