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Thioredoxin Antibody (YA035)

(Synonyms: TXN; ADF; ATL-derived factor; SASP; Thioredoxin; TRX1; Thioredoxin delta 3; TRX; TXN delta 3; TRDX)
Customer Review

Based on 1 Customer Validation

Thioredoxin Antibody (YA035) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thioredoxin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
  • Thioredoxin Antibody (YA035) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:10000 1:50-1:200 1:50-1:100 1:100-1:500 1:20

Product Details

Description

Thioredoxin Antibody (YA035) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thioredoxin.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 12 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 12 kDa
Species Reactivity Database

Entrez Gene: 7295 Human

SwissProt: P10599 Human

Immunogen

Synthetic peptide corresponding to Human Thioredoxin/TRX.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102749

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Thioredoxin Antibody (YA035)
    Western blot analysis of extracts from Jurkat(lane 2(20μg) and Jurkat(lane 3(40μg), Hela(lane 4(20μg) ) and Hela(lane 5(40μg) ) using Thioredoxin (HY-P80912) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80912, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), U-87MG (lane 2, 15 μg), SW480 (lane 3, 15 μg), and HT-1080 (lane 4, 15 μg) using Thioredoxin / TRX Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
    Immunocytochemistry analysis of Hela cells labeling Thioredoxin with Thioredoxin Antibody (HY-P80912) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Thioredoxin Antibody (HY-P80912) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
    Immunocytochemistry analysis of Hela cells labeling Thioredoxin with Thioredoxin Antibody (HY-P80912) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Thioredoxin Antibody (HY-P80912) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (PubMed:17182577, PubMed:19032234, PubMed:2176490). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity (PubMed:16408020, PubMed:17606900). Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (PubMed:11118054, PubMed:9108029); ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55)

  • Subcellular Localization

    Nucleus; Cytoplasm; Secreted

  • Expression


    Induction:Up-regulated by ionizing radiation

  • Isoforms & Post-Translational Modification

    P10599 has 2 isomers: P10599-1: 11737 Da (predicted); P10599-2: 9452 Da (predicted).
    In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins;In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation

  • Subunit

    Homodimer; disulfide-linked (PubMed:17260951, PubMed:9369469). Interacts with TXNIP through the redox-active site (PubMed:17260951). Interacts with MAP3K5 and CASP3 (PubMed:15246877). In case of infection, interacts with S.typhimurium protein slrP (PubMed:19690162). Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA-binding activity in a redox-dependent manner (PubMed:9108029)

  • SwissProt ID

    P10599

  • Gene ID
    7295 [NCBI]
  • Synonyms

    TXN; ADF; ATL-derived factor; SASP; Thioredoxin; TRX1; Thioredoxin delta 3; TRX; TXN delta 3; TRDX

  • Research Field

    Tags & Cell Markers

Thioredoxin Antibody (YA035) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20μg) and Jurkat(lane 3(40μg), Hela(lane 4(20μg) ) and Hela(lane 5(40μg) ) using Thioredoxin (HY-P80912) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80912, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), U-87MG (lane 2, 15 μg), SW480 (lane 3, 15 μg), and HT-1080 (lane 4, 15 μg) using Thioredoxin / TRX Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Thioredoxin with Thioredoxin Antibody (HY-P80912) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Thioredoxin Antibody (HY-P80912) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Thioredoxin with Thioredoxin Antibody (HY-P80912) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Thioredoxin Antibody (HY-P80912) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Thioredoxin Antibody (YA035) Product Image
    1/16
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
  • Thioredoxin Antibody (YA035) Product Image
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20μg) and Jurkat(lane 3(40μg), Hela(lane 4(20μg) ) and Hela(lane 5(40μg) ) using Thioredoxin (HY-P80912) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80912, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), U-87MG (lane 2, 15 μg), SW480 (lane 3, 15 μg), and HT-1080 (lane 4, 15 μg) using Thioredoxin / TRX Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Thioredoxin with Thioredoxin Antibody (HY-P80912) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Thioredoxin Antibody (HY-P80912) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Thioredoxin with Thioredoxin Antibody (HY-P80912) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Thioredoxin Antibody (HY-P80912) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
  • Experimental Validation Results for Thioredoxin Antibody (YA035)
  • Thioredoxin Antibody (YA035) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Jurkat(lane 2(20μg) and Jurkat(lane 3(40μg), Hela(lane 4(20μg) ) and Hela(lane 5(40μg) ) using Thioredoxin (HY-P80912) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80912, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Thioredoxin Antibody (YA035) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), U-87MG (lane 2, 15 μg), SW480 (lane 3, 15 μg), and HT-1080 (lane 4, 15 μg) using Thioredoxin / TRX Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Thioredoxin Antibody (YA035) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Thioredoxin with Thioredoxin Antibody (HY-P80912) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Thioredoxin Antibody (HY-P80912) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Thioredoxin Antibody (YA035) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Thioredoxin with Thioredoxin Antibody (HY-P80912) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Thioredoxin Antibody (HY-P80912) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Thioredoxin Antibody (YA035) Product Image WB
  • Thioredoxin Antibody (YA035) Product Image WB
  • Thioredoxin Antibody (YA035) Product Image ICC
  • Thioredoxin Antibody (YA035) Product Image ICC