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DOCK8 Antibody (YA2713)

(Synonyms: MRD2; ZIR8; HEL-205)
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DOCK8 Antibody (YA2713) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DOCK8.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • DOCK8 Antibody (YA2713) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

DOCK8 Antibody (YA2713) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DOCK8.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 239 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 239 kDa
Species Reactivity Database

Entrez Gene: 81704 Human

SwissProt: Q8NF50 Human

Immunogen

Recombinant protein of human DOCK8

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    DOCK8 is a Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP. During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC. Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane. Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing[1][2][3].

  • Subcellular Localization

    Cytoplasm; Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side

  • Expression


    Tissue_specificity:Expressed in peripheral blood mononuclear cells (PBMCs)

  • Isoforms & Post-Translational Modification

    Q8NF50 has 4 isomers: Q8NF50-1: 238529 Da (predicted); Q8NF50-2: 235185 Da (predicted); Q8NF50-3: 230820 Da (predicted); Q8NF50-4: 227477 Da (predicted).
    In response to chemokine CXCL12/SDF-1-alpha stimulation, phosphorylated by PRKCA/PKC-alpha which promotes DOCK8 dissociation from LRCH1

  • Subunit

    Interacts (via DOCKER domain) with GTPase CDC42; the interaction activates CDC42 by exchanging GDP for GTP (PubMed:22461490, PubMed:28028151). The unphosphorylated form interacts (via DOCKER domain) with LRCH1 (via LRR repeats); the interaction prevents the association between DOCK8 and CDC42 (PubMed:28028151). Interacts with CCDC88B (PubMed:25762780)

  • SwissProt ID

    Q8NF50

  • Gene ID
    81704 [NCBI]
  • Synonyms

    MRD2; ZIR8; HEL-205

  • Research Field

    Cardiovascular

DOCK8 Antibody (YA2713) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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