DOCK8 Antibody (YA2713)

Cat. No.: HY-P82968: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

DOCK8 Antibody (YA2713) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DOCK8.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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DOCK8 Antibody (YA2713) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

DOCK8 Antibody (YA2713) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DOCK8.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 239 kDa;

Observed band size: 239 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q8NF50

Gene ID

81704 [NCBI]

Immunogen

Recombinant protein of human DOCK8

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP (PubMed:22461490, PubMed:28028151). During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (By similarity). Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane (PubMed:28028151). Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing (PubMed:25762780)

Subcellular Localization：Cytoplasm; Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side

Expression：
Tissue_specificity:Expressed in peripheral blood mononuclear cells (PBMCs)

Isoforms & Post-Translational Modification：Q8NF50 has 4 isomers: Q8NF50-1: 238529 Da (predicted); Q8NF50-2: 235185 Da (predicted); Q8NF50-3: 230820 Da (predicted); Q8NF50-4: 227477 Da (predicted).
In response to chemokine CXCL12/SDF-1-alpha stimulation, phosphorylated by PRKCA/PKC-alpha which promotes DOCK8 dissociation from LRCH1

Subunit：Interacts (via DOCKER domain) with GTPase CDC42; the interaction activates CDC42 by exchanging GDP for GTP (PubMed:22461490, PubMed:28028151). The unphosphorylated form interacts (via DOCKER domain) with LRCH1 (via LRR repeats); the interaction prevents the association between DOCK8 and CDC42 (PubMed:28028151). Interacts with CCDC88B (PubMed:25762780)

Database

Entrez Gene: 81704 Human

SwissProt: Q8NF50 Human

OMIM: 243700 Human

Research Field

Cardiovascular

Synonyms

MRD2; ZIR8; HEL-205

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

DOCK8 Antibody (YA2713) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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DOCK8 Antibody (YA2713)

DOCK8 Antibody (YA2713) Featured Recommendations:

Product Detail

Background

Documentation

DOCK8 Antibody (YA2713) Related Classifications

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