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TIM3 Antibody (YA1448)

(Synonyms: CD366 antibody; FLJ14428 antibody; HAVcr-2 antibody; Havcr2 antibody; HAVR2_HUMAN antibody; Hepatitis A virus cellular receptor 2 antibody; Kidney injury molecule 3 antibody; KIM 3 antibody; KIM3 antibody; T cell immunoglobulin and mucin domain containing 3 antibody; T cell immunoglobulin mucin 3 antibody; T-cell immunoglobulin and mucin domain-containing protein 3 antibody; T-cell immunoglobulin mucin family member 3 antibody; T-cell immunoglobulin mucin receptor 3 antibody; T-cell membrane protein 3 antibody; Tim 3 antibody; TIM-3 antibody; TIM3 antibody; TIMD-3 antibody; TIMD3 antibody; )
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Based on 1 publication(s) in Google Scholar

TIM3 Antibody (YA1448) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to TIM3.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for TIM3 Antibody (YA1448)
  • Experimental Validation Results for TIM3 Antibody (YA1448)
  • Experimental Validation Results for TIM3 Antibody (YA1448)
  • TIM3 Antibody (YA1448) Product Image
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:2000 1:50-1:200 1:1000-1:5000 1:50-1:100

Product Details

Description

TIM3 Antibody (YA1448) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to TIM3.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 33 kDa
Species Reactivity Database

Entrez Gene: 84868 Human

SwissProt: Q8TDQ0 Human

Immunogen

Recombinant protein with Human Tim3 1-89 / 301.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103810

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TIM3 Antibody (YA1448)
    Western blot analysis of extracts from A549(lane 2(20ug) , HepG2(lane 3(20ug) and HEK293(lane 4(20ug) using TIM3 Antibody (HY-P81703) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TIM3 Antibody (YA1448)
    Immunocytochemistry analysis of 293T cells labeling TIM3 Antibody (HY-P81703) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with TIM3 Antibody (HY-P81703) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TIM3 Antibody (YA1448)
    Immunocytochemistry analysis of 293T cells labeling TIM3 Antibody (HY-P81703) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with TIM3 Antibody (HY-P81703) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    CD366 (TIM-3) is a cell-surface receptor involved in regulating innate and adaptive immune responses. CD366 (TIM-3) regulates macrophage activation, inhibits Type 1 helper T cell (Th1)-mediated autoimmune and alloimmune responses, and promotes immune tolerance. In CD8+ cells, CD366 (TIM-3) attenuates T-cell receptor (TCR)-induced signaling, leading to reduced IL-2 secretion by blocking NF-κB and NFAT promoter activity.

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell junction; Cell membrane

  • Expression


    Tissue_specificity:Expressed in T-helper type 1 (Th1) lymphocytes. Expressed on regulatory T (Treg) cells after TCR stimulation. Expressed in dendritic cells and natural killer (NK) cells. Expressed in epithelial tissues. Expression is increased on CD4+ and CD8+ T-cells in chronic hepatitis C virus (HCV) infection. In progressive HIV-1 infection, expression is up-regulated on HIV-1-specific CD8 T-cells.

  • Isoforms & Post-Translational Modification

    CD366 (TIM-3) has 2 isomers: Q8TDQ0-1: 33394 Da (predicted); Q8TDQ0-2: 16149 Da (predicted).
    O-glycosylated with core 1 or possibly core 8 glycans;Phosphorylated on tyrosine residues; modestly increased after TCR/CD28 stimulation. Can be phosphorylated in the cytoplasmic domain by FYN (By similarity). Phosphorylation at Tyr-265 is increased by stimulation with ligand LGALS9;Palmitoylated by ZDHHC9 at Cys-296; palmitoylation stabilizes HAVCR2 by preventing binding to E3 ubiquitin ligase SYVN1, thereby suppressing its polyubiquitination and degradation;Ubiquitinated by SYVN1, leading to polyubiquitination and proteasomal degradation

  • Subunit

    Interacts (in basal state T-cells) with VAV1; AKT1/2, LCP2, ZAP70, SYK, PIK3R1, FYN, SH3BP2 and SH2D2A. Interacts (in activated T-cells) with LCK and PLCG.

  • SwissProt ID

    Q8TDQ0

  • Gene ID
    84868 [NCBI]
  • Synonyms

    CD366 antibody; FLJ14428 antibody; HAVcr-2 antibody; Havcr2 antibody; HAVR2_HUMAN antibody; Hepatitis A virus cellular receptor 2 antibody; Kidney injury molecule 3 antibody; KIM 3 antibody; KIM3 antibody; T cell immunoglobulin and mucin domain containing 3 antibody; T cell immunoglobulin mucin 3 antibody; T-cell immunoglobulin and mucin domain-containing protein 3 antibody; T-cell immunoglobulin mucin family member 3 antibody; T-cell immunoglobulin mucin receptor 3 antibody; T-cell membrane protein 3 antibody; Tim 3 antibody; TIM-3 antibody; TIM3 antibody; TIMD-3 antibody; TIMD3 antibody;

  • Research Field

    Immunology

TIM3 Antibody (YA1448) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20ug) , HepG2(lane 3(20ug) and HEK293(lane 4(20ug) using TIM3 Antibody (HY-P81703) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of 293T cells labeling TIM3 Antibody (HY-P81703) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with TIM3 Antibody (HY-P81703) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of 293T cells labeling TIM3 Antibody (HY-P81703) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with TIM3 Antibody (HY-P81703) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TIM3 Antibody (YA1448) Product Image
    1/16
  • Experimental Validation Results for TIM3 Antibody (YA1448)
  • Experimental Validation Results for TIM3 Antibody (YA1448)
  • Experimental Validation Results for TIM3 Antibody (YA1448)
  • TIM3 Antibody (YA1448) Product Image
  • Experimental Validation Results for TIM3 Antibody (YA1448)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20ug) , HepG2(lane 3(20ug) and HEK293(lane 4(20ug) using TIM3 Antibody (HY-P81703) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TIM3 Antibody (YA1448)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of 293T cells labeling TIM3 Antibody (HY-P81703) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with TIM3 Antibody (HY-P81703) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TIM3 Antibody (YA1448)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of 293T cells labeling TIM3 Antibody (HY-P81703) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with TIM3 Antibody (HY-P81703) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TIM3 Antibody (YA1448)
  • Experimental Validation Results for TIM3 Antibody (YA1448)
  • Experimental Validation Results for TIM3 Antibody (YA1448)
  • TIM3 Antibody (YA1448) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from A549(lane 2(20ug) , HepG2(lane 3(20ug) and HEK293(lane 4(20ug) using TIM3 Antibody (HY-P81703) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • TIM3 Antibody (YA1448) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of 293T cells labeling TIM3 Antibody (HY-P81703) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with TIM3 Antibody (HY-P81703) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TIM3 Antibody (YA1448) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of 293T cells labeling TIM3 Antibody (HY-P81703) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with TIM3 Antibody (HY-P81703) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TIM3 Antibody (YA1448) Product Image WB
  • TIM3 Antibody (YA1448) Product Image ICC
  • TIM3 Antibody (YA1448) Product Image ICC