Fas(C-term) Antibody (YA5207)

Cat. No.: HY-P85515: Data Sheet User Guide for Antibodies
FAQs
Technical Support

Fas(C-term) Antibody (YA5207) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Fas(C-term).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Fas(C-term) Antibody (YA5207) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Fas(C-term).

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Observed band size: 45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P25445

Gene ID

355 [NCBI]

Immunogen

Purified recombinant human FAS (C-terminus) protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Membrane raft; Secreted; Secreted; Secreted; Secreted; Secreted

Expression：
Tissue_specificity:Subtype 1 and subtype 6 were expressed at equal levels in resting peripheral blood mononuclear cells. Upon activation, subtype 1 levels increased, while subtype 6 levels decreased.

Isoforms & Post-Translational Modification：P25445 has 7 isomers: P25445-1: 37732 Da (predicted); P25445-2: 11435 Da (predicted); P25445-3: 9390 Da (predicted); P25445-4: 16648 Da (predicted); P25445-5: 14602 Da (predicted); P25445-6: 35386 Da (predicted); P25445-7: 24781 Da (predicted).
(Microbial infection) Glycosylated at Arg-250 by enteropathogenic E.coli protein NleB1: arginine GlcNAcylation prevents homotypic/heterotypic death domain interactions;Palmitoylated (PubMed:25301068). Palmitoylation by ZDHHC7 prevents the lysosomal degradation of FAS regulating its expression at the plasma membrane (PubMed:25301068);N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans

Subunit：Component of the death-induced signaling complex (DISC) composed of cell surface receptor FAS/CD95, adapter protein FADD and the CASP8 protease; recruitment of CASP8 to the complex is required for processing of CASP8 into the p18 and p10 subunits (PubMed:21109225, PubMed:9184224, PubMed:9322534).

Synonyms

ALPS 1A; ALPS1A; APO 1; Apo 1 antigen; APO 1 cell surface antigen; Apo-1 antigen; APO1; Apo1 antigen; APO1 cell surface antigen; Apoptosis antigen 1; Apoptosis mediating surface antigen FAS; Apoptosis-mediating surface antigen FAS; APT 1; APT1; CD 95; CD 95 antigen; CD95; CD95 antigen; Delta Fas; Delta Fas/APO 1/CD95; Delta Fas/APO1/CD95; Fas; TNF receptor superfamily, member 6; FAS 1; FAS 827dupA; Fas AMA; FAS; FAS Antigen; Fas cell surface death receptor; FAS1; FASLG receptor; FASTM; sFAS; Surface antigen APO1; TNF receptor superfamily, member 6; TNFRSF 6; TNFRSF6; TNR6_HUMAN; Tumor necrosis factor receptor superfamily member 6.

Documentation

User Guide for Antibodies (1077 KB)