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MCP1 Antibody (YA3779)

(Synonyms: HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30)
Customer Review

Based on 1 Customer Validation

MCP1 Antibody (YA3779) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MCP1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL IHC-P ICC
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • MCP1 Antibody (YA3779) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

MCP1 Antibody (YA3779) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MCP1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 11 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 11 kDa
Immunogen

Purified recombinant fragment of human CCL2 full(aa 1-99).

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718829

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MCP1 Antibody (YA3779)
    Immunohistochemical analysis of paraffin-embedded human testis tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MCP1 Antibody (YA3779)
    Immunohistochemical analysis of paraffin-embedded rat testis tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MCP1 Antibody (YA3779)
    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MCP1 Antibody (YA3779)
    Immunohistochemical analysis of paraffin-embedded human Colorectal cancer 1 tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MCP1 Antibody (YA3779)
    Immunohistochemical analysis of paraffin-embedded human Colorectal cancer 2 tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MCP1 Antibody (YA3779)
    Immunohistochemical analysis of paraffin-embedded human Colorectal cancer 3 tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MCP1 Antibody (YA3779)
    Immunocytochemistry analysis of HepG2 cells labeling MCP1 with MCP1 Antibody (HY-P84082) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MCP1 Antibody (HY-P84082) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MCP1 Antibody (YA3779)
    Immunocytochemistry analysis of U2OS cells labeling MCP1 with MCP1 Antibody (HY-P84082) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MCP1 Antibody (HY-P84082) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    MCP1 acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis[1][2][3][4][5][6][7].

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Expression (protein level) in seminal plasma, endometrial fluid, and follicular fluid (PubMed: 23765988) . Expression in monocytes (PubMed: 2513477) .

    Induction:Up-regulated upon hypertonic conditions (PubMed:23233732) . In pancreatic islets, secretion is stimulated by IL1B (PubMed:23955712) . Up-regulated by coagulation factor Xa (F10) in PAR-1 (F2R) -dependent manner in cardiac fibroblasts and endothelial cells (PubMed:30568593, PubMed:34831181) . Up-regulated by thrombin (F2) in endothelial cells (PubMed:30568593)

  • Subunit

    Monomer or homodimer; in equilibrium (PubMed:15033992, PubMed:8898111, PubMed:8989326, PubMed:9837883). Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans (PubMed:9792674). Interacts with TNFAIP6 (via Link domain)

  • SwissProt ID

    P13500

  • Gene ID
    6347 [NCBI]
  • Synonyms

    HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30

References

[1]. Hemmerich S, et al. Identification of residues in the monocyte chemotactic protein-1 that contact the MCP-1 receptor, CCR2. Biochemistry. 1999 Oct 5;38(40):13013-25. [Content Brief]

[2]. Jarnagin K, et al. Identification of surface residues of the monocyte chemotactic protein 1 that affect signaling through the receptor CCR2. Biochemistry. 1999 Dec 7;38(49):16167-77. [Content Brief]

[3]. Paavola CD, et al. Monomeric monocyte chemoattractant protein-1 (MCP-1) binds and activates the MCP-1 receptor CCR2B. J Biol Chem. 1998 Dec 11;273(50):33157-65. [Content Brief]

[4]. Zhang YJ, et al. Structure/activity analysis of human monocyte chemoattractant protein-1 (MCP-1) by mutagenesis. Identification of a mutated protein that inhibits MCP-1-mediated monocyte chemotaxis. J Biol Chem. 1994 Jun 3;269(22):15918-24. [Content Brief]

[5]. Weber M, et al. Deletion of the NH2-terminal residue converts monocyte chemotactic protein 1 from an activator of basophil mediator release to an eosinophil chemoattractant. J Exp Med. 1996 Feb 1;183(2):681-5. [Content Brief]

[6]. Chakravarty L, et al. Lysine 58 and histidine 66 at the C-terminal alpha-helix of monocyte chemoattractant protein-1 are essential for glycosaminoglycan binding. J Biol Chem. 1998 Nov 6;273(45):29641-7. [Content Brief]

[7]. Li YS, et al. The expression of monocyte chemotactic protein (MCP-1) in human vascular endothelium in vitro and in vivo. Mol Cell Biochem. 1993 Sep 8;126(1):61-8. [Content Brief]

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MCP1 Antibody (YA3779) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat testis tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testis tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colorectal cancer 1 tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colorectal cancer 2 tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colorectal cancer 3 tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling MCP1 with MCP1 Antibody (HY-P84082) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MCP1 Antibody (HY-P84082) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of U2OS cells labeling MCP1 with MCP1 Antibody (HY-P84082) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MCP1 Antibody (HY-P84082) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MCP1 Antibody (YA3779) Product Image
    1/16
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • MCP1 Antibody (YA3779) Product Image
  • Experimental Validation Results for MCP1 Antibody (YA3779)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MCP1 Antibody (YA3779)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat testis tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MCP1 Antibody (YA3779)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testis tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MCP1 Antibody (YA3779)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colorectal cancer 1 tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MCP1 Antibody (YA3779)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colorectal cancer 2 tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MCP1 Antibody (YA3779)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colorectal cancer 3 tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MCP1 Antibody (YA3779)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling MCP1 with MCP1 Antibody (HY-P84082) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MCP1 Antibody (HY-P84082) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MCP1 Antibody (YA3779)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of U2OS cells labeling MCP1 with MCP1 Antibody (HY-P84082) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MCP1 Antibody (HY-P84082) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • Experimental Validation Results for MCP1 Antibody (YA3779)
  • MCP1 Antibody (YA3779) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human testis tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MCP1 Antibody (YA3779) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat testis tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MCP1 Antibody (YA3779) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MCP1 Antibody (YA3779) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colorectal cancer 1 tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MCP1 Antibody (YA3779) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colorectal cancer 2 tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MCP1 Antibody (YA3779) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colorectal cancer 3 tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MCP1 Antibody (YA3779) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling MCP1 with MCP1 Antibody (HY-P84082) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MCP1 Antibody (HY-P84082) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MCP1 Antibody (YA3779) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of U2OS cells labeling MCP1 with MCP1 Antibody (HY-P84082) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with MCP1 Antibody (HY-P84082) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MCP1 Antibody (YA3779) Product Image IHC-P
  • MCP1 Antibody (YA3779) Product Image IHC-P
  • MCP1 Antibody (YA3779) Product Image IHC-P
  • MCP1 Antibody (YA3779) Product Image IHC-P
  • MCP1 Antibody (YA3779) Product Image IHC-P
  • MCP1 Antibody (YA3779) Product Image IHC-P
  • MCP1 Antibody (YA3779) Product Image ICC
  • MCP1 Antibody (YA3779) Product Image ICC