MCP1 Antibody (YA3779)

Cat. No.: HY-P84082: Data Sheet COA
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MCP1 Antibody (YA3779) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MCP1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

MCP1 Antibody (YA3779) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MCP1.

Host

Mouse

Clonality

Monoclonal Antibody

Molecular Weight

Predicted band size: 11 kDa;

Observed band size: 11 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Monkey

SwissProt ID

P13500

Gene ID

6347 [NCBI]

Immunogen

Purified recombinant fragment of human CCL2 full(aa 1-99).

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FCM	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

IHC-P

Immunohistochemical analysis of paraffin-embedded human testis tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat testis tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human Colorectal cancer 1 tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human Colorectal cancer 2 tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human Colorectal cancer 3 tissue using MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84082, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Acts as a ligand for C-C chemokine receptor CCR2 (PubMed:10529171, PubMed:10587439, PubMed:9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:10587439, PubMed:9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed:8195247, PubMed:8627182, PubMed:9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed:8107690)

Subcellular Localization：Secreted

Expression：
Tissue_specificity:Expression (protein level) in seminal plasma, endometrial fluid, and follicular fluid (PubMed: 23765988) . Expression in monocytes (PubMed: 2513477) .

Induction:Up-regulated upon hypertonic conditions (PubMed:23233732) . In pancreatic islets, secretion is stimulated by IL1B (PubMed:23955712) . Up-regulated by coagulation factor Xa (F10) in PAR-1 (F2R) -dependent manner in cardiac fibroblasts and endothelial cells (PubMed:30568593, PubMed:34831181) . Up-regulated by thrombin (F2) in endothelial cells (PubMed:30568593)

Subunit：Monomer or homodimer; in equilibrium (PubMed:15033992, PubMed:8898111, PubMed:8989326, PubMed:9837883). Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans (PubMed:9792674). Interacts with TNFAIP6 (via Link domain)

RRID

AB_3718829

Synonyms

HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)