MBD3 Antibody (YA1695)
(Synonyms: MBD3; Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3)
Based on 1 Customer Validation
MBD3 Antibody (YA1695) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MBD3.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, FC, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|
| Dilution Ratio | 1:1000-1:2000 | 1:50-1:200 | 1:50-1:100 | 1:50 |
Product Details
MBD3 Antibody (YA1695) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MBD3.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 33 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 33 kDa
Entrez Gene: 53615 Human ; 17192 Mouse ; 362834 Rat
SwissProt: O95983 Human ; Q9Z2D8 Mouse ;
OMIM: 603573 Human
A synthesized peptide derived from human MBD3 aa230-291/291.
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MBD3 acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to methylated DNA by itself. Binds to a lesser degree DNA containing unmethylated CpG dinucleotides. Recruits histone deacetylases and DNA methyltransferases[1][2][3][4][5][6].
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Subcellular Localization
Nucleus; Chromosome
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Isoforms & Post-Translational Modification
O95983 has 2 isomers: O95983-1: 32844 Da (predicted); O95983-2: 29014 Da (predicted).
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Subunit
Heterodimer (via N-terminus) with MBD2 (PubMed:10947852, PubMed:15701600). Component of the MeCP1 histone deacetylase complex (PubMed:11756549, PubMed:12124384).
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SwissProt ID
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Synonyms
MBD3; Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3
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Research Field
Epigenetics and Nuclear Signaling
Documentation
[1]. Saito M, et al. The mCpG-binding domain of human MBD3 does not bind to mCpG but interacts with NuRD/Mi2 components HDAC1 and MTA2. J Biol Chem. 2002 Sep 20;277(38):35434-9. [Content Brief]
[2]. Le Guezennec X, et al. MBD2/NuRD and MBD3/NuRD, two distinct complexes with different biochemical and functional properties. Mol Cell Biol. 2006 Feb;26(3):843-51. [Content Brief]
[3]. Hoffmeister H, et al. CHD3 and CHD4 form distinct NuRD complexes with different yet overlapping functionality. Nucleic Acids Res. 2017 Oct 13;45(18):10534-10554. [Content Brief]
[4]. Tatematsu KI, et al. MBD2-MBD3 complex binds to hemi-methylated DNA and forms a complex containing DNMT1 at the replication foci in late S phase. Genes Cells. 2000 Aug;5(8):677-88. [Content Brief]
[5]. Morey L, et al. MBD3, a component of the NuRD complex, facilitates chromatin alteration and deposition of epigenetic marks. Mol Cell Biol. 2008 Oct;28(19):5912-23. [Content Brief]
[6]. Cramer JM, et al. Probing the dynamic distribution of bound states for methylcytosine-binding domains on DNA. J Biol Chem. 2014 Jan 17;289(3):1294-302. [Content Brief]
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