NCK1 Antibody (YA3294)(PBS only)

Art. -Nr.: HY-P83549A: Data Sheet User Guide for Antibodies
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NCK1 Antibody (YA3294) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NCK1.

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100 μL		Angebot einholen

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung
Verweise

Beschreibung

NCK1 Antibody (YA3294) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NCK1.

Host

Rabbit

Molekulargewicht

Predicted band size: 43 kDa;

Observed band size: 43 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P16333

Gene ID

4690 [NCBI]

Immunogen

A synthetic peptide of human Nck

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:200
FC FC: Flow Cytometry	1:200-1:1000

Reinheit	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors

Subcellular Localization：Cytoplasm; Endoplasmic reticulum; Nucleus

Isoforms & Post-Translational Modification：P16333 has 2 isomers: P16333-1: 42864 Da (predicted); P16333-2: 35511 Da (predicted).
Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated by activated PDGFRB

Subunit：Interacts (via SH2 domain and SH3 domain 2) with EGFR. Interacts with PAK1 and SOS1. Interacts (via SH3 domains) with PKN2. Associates with BLNK, PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with SOCS7. This interaction is required for nuclear import. Part of a complex containing PPP1R15B, PP1 and NCK1. Interacts with RALGPS1. Interacts with CAV2 (tyrosine phosphorylated form). Interacts with ADAM15. Interacts with FASLG. Directly interacts with RASA1. Interacts with isoform 4 of MINK1. Interacts with FLT1 (tyrosine phosphorylated). Interacts with KDR (tyrosine phosphorylated). Interacts (via SH2 domain) with EPHB1; activates the JUN cascade to regulate cell adhesion. Interacts with EPHA2. Interacts (via SH2 domain) with PDGFRB (tyrosine phosphorylated). Interacts with the inactive form of EIF2AK2/PKR. Interacts with PTPN1 (PubMed:21707536). Interacts with INSR/insulin receptor (in response to insulin stimulation); This interaction may mediate PTPN1 recruitment leading to INSR dephosphorylation (PubMed:21707536). Interacts with IRS1 (PubMed:8265614)

Research Field

Signal Transduction

Synonyms

NCK; nck-1; NCKalpha

Dokumentation

User Guide for Antibodies (1077 KB)

Verweise

[1]. /biology-dictionary/mt4-cell.html [Content Brief]