NTH1 Antibody (YA1435)(PBS only)

Cat. No.: HY-P81690A: Data Sheet User Guide for Antibodies
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NTH1 Antibody (YA1435) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NTH1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

NTH1 Antibody (YA1435) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NTH1.

Host

Rabbit

Molecular Weight

Predicted band size: 34 kDa;

Observed band size: 34 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P78549

Gene ID

4913 [NCBI]

Immunogen

Recombinant protein of human NTH1 aa24-126.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage (PubMed:29610152, PubMed:9927729). The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines. Also has 8-oxo-7,8-dihydroguanine (8-oxoG) DNA glycosylase activity. Acts preferentially on DNA damage opposite guanine residues in DNA. Is able to process lesions in nucleosomes without requiring or inducing nucleosome disruption

Subcellular Localization：Nucleus; Mitochondrion

Expression：
Tissue_specificity:Widely expressed with highest levels in heart and lowest levels in lung and liver

Isoforms & Post-Translational Modification：P78549 has 3 isomers: P78549-2: 33570 Da (predicted); P78549-1: 34390 Da (predicted); P78549-3: 32839 Da (predicted).
Ubiquitinated by TRIM26; leading to proteasomal degradation

Subunit：Interacts with YBX1 (By similarity) (PubMed:11287425). Interacts with ERCC5/XPG; the interaction stimulates NTHL1 activity and NTHL1 binding to its DNA substrate (PubMed:9927729)

Research Field

Epigenetics and Nuclear Signaling

Synonyms

FAP3; NTH1; OCTS3; hNTH1

Documentation

User Guide for Antibodies (1077 KB)