MYH9 Antibody (YA3113)(PBS only)

Art. -Nr.: HY-P83368A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

MYH9 Antibody (YA3113) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MYH9.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Größe		Verfügbarkeit
50 μL		Angebot einholen
100 μL		Angebot einholen

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung

Beschreibung

MYH9 Antibody (YA3113) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MYH9.

Host

Rabbit

Molekulargewicht

Predicted band size: 227 kDa;

Observed band size: 227 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

P35579

Gene ID

4627 [NCBI]

Immunogen

A synthetic peptide of human non-muscle Myosin IIA

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:20

Reinheit	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Required for cortical actin clearance prior to oocyte exocytosis (By similarity). Promotes cell motility in conjunction with S100A4 (PubMed:16707441). During cell spreading, plays an important role in cytoskeleton reorganization, focal contact formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10 (PubMed:20052411); (Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1 envelope glycoprotein B

Subcellular Localization：Cytoplasm, cytoskeleton; Cytoplasm, cell cortex; Cytoplasmic vesicle, secretory vesicle, Cortical granule; Cell membrane; Cell membrane

Expression：
Tissue_specificity:In the kidney, expressed in the glomeruli. Also expressed in leukocytes

Isoforms & Post-Translational Modification：P35579 has 2 isomers: P35579-1: 226532 Da (predicted); P35579-2: 159864 Da (predicted).
ISGylated;Ubiquitination

Subunit：Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with RASIP1 (By similarity). Interacts with DDR1 (By similarity). Interacts with PDLIM2 (By similarity). Interacts with SVIL (PubMed:12917436, PubMed:17925381). Interacts with HTRA3 (PubMed:22229724). Interacts with Myo7a (By similarity). Interacts with CFAP95 (PubMed:28345668). Interacts with LIMCH1; independently of the integration of MYH9 into the myosin complex (PubMed:28228547). Interacts with RAB3A (PubMed:27325790). Interacts with ZBED4 (PubMed:22693546). Interacts with S100A4; this interaction increases cell motility (PubMed:16707441)

Research Field

Signal Transduction

Synonyms

MHA; FTNS; EPSTS; BDPLT6; DFNA17; MATINS; NMMHCA; NMHC-II-A; NMMHC-IIA

Dokumentation

User Guide for Antibodies (1077 KB)