LTBR Antibody (YA3985)

Cat. No.: HY-P84288: Ficha de datos COA
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LTBR Antibody (YA3985) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to LTBR.

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Tamaño	Stock	Cantidad
20 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Descripciòn

Descripciòn

LTBR Antibody (YA3985) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to LTBR.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 47 kDa;

Observed band size: 47 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P36941

Gene ID

4055 [NCBI]

Immunogen

Purified recombinant fragment of human LTBR (AA: extra 31-227) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureza	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

ALL WB FC

Western blot analysis of extracts from HepG2(lane2(20μg), NIH/3T3(lane3(20μg), A431(lane4(20μg) and Jurkat(lane5(20μg) using LTBR Antibody (HY-P84288). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
Flow cytometric analysis of 1X10⁶ HeLa cells labeling LTBR Antibody(HY-P84288, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

Function：Receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT (PubMed:24248355). Activates NF-kappa-B signaling pathway upon stimulation with lymphotoxin (LTA(1)-LTB(2)) (PubMed:24248355). Promotes apoptosis via TRAF3 and TRAF5. May play a role in the development of lymphoid organs

Subcellular Localization：Membrane; Single-pass type I membrane protein

Isoforms & Post-Translational Modification：P36941 has 2 isomers: P36941-1: 46709 Da (predicted); P36941-2: 44810 Da (predicted).

Subunit：Self-associates; dimerization and trimerization are promoted by lymphotoxin (LTA(1)-LTB(2)) (PubMed:29507355). Associates with TRAF3 (PubMed:8663299). Associates with TRAF4 (PubMed:9626059). Associates with TRAF5 (PubMed:9511754). Interacts with Aedes aegypti lymphotoxin beta receptor inhibitor; the interaction reduces dimerization and trimerization of LTBR induced by lymphotoxin (LTA(1)-LTB(2)) (PubMed:29507355, PubMed:38248473)

Synonyms

TNFCR; TNFR3; D12S370; TNFR-RP; TNFRSF3; TNFR2-RP; LT-BETA-R; TNF-R-III

Documentación

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Ficha de datos (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)