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IFN gamma Antibody (YA3520)

(Synonyms: IFNG; IFG; IFI; IFN Gamma; IFN Immune; IFN-gamma; IFNG; IFNG_HUMAN; Immune Interferon; Interferon gamma; Interferon Gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon.)
2 Cited Publications
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Based on 2 publication(s) in Google Scholar

IFN gamma Antibody (YA3520) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IFN gamma.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for IFN gamma Antibody (YA3520)
  • Experimental Validation Results for IFN gamma Antibody (YA3520)
  • IFN gamma Antibody (YA3520) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50-1:100

Product Details

Description

IFN gamma Antibody (YA3520) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IFN gamma.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 25 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 19 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Interferon gamma aa25-50.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103216

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IFN gamma Antibody (YA3520)
    Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using IFN gamma Antibody (HY-P81111) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IFN gamma Antibody (YA3520)
    Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), Mouse spleen (lane 2, 15 μg), Rat spleen (lane 3, 15 μg), Mouse thymus (lane 4, 15 μg), and Rat thymus (lane 5, 15 μg) using IFN gamma Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    IFN-γ (Interferon-gamma) is a key immune cytokine produced by NK cells, Th1 cells, and CD8⁺ T cells. It regulates macrophage activation, Th1 differentiation, antigen presentation, and anti-tumor immunity via the IFNGR-JAK-STAT1 pathway. IFN-γ serves as an important indicator for assessing cellular immune function, T-cell activation status, and the efficacy of immunotherapy.

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Mainly released by activated T lymphocytes

    Induction:By cytokines, most notably interleukin IL-12, secreted by professional antigen-presenting cells such as monocytes/macrophages and dendritic cells

  • Subunit

    Homodimer. Interacts with IFNGR1 (via extracellular domain); this interaction promotes IFNGR1 dimerization

  • SwissProt ID

    P01579

  • Gene ID
    3458 [NCBI]
  • Synonyms

    IFNG; IFG; IFI; IFN Gamma; IFN Immune; IFN-gamma; IFNG; IFNG_HUMAN; Immune Interferon; Interferon gamma; Interferon Gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon.

References

[1]. El Bougrini J, et al. Arsenic enhances the apoptosis induced by interferon gamma: key role of IRF-1. Cell Mol Biol (Noisy-le-grand). 2006 May 15;52(1):9-15. [Content Brief]

[2]. Hisamatsu H, et al. Newly identified pair of proteasomal subunits regulated reciprocally by interferon gamma. J Exp Med. 1996 Apr 1;183(4):1807-16. [Content Brief]

[3]. Greenlund AC, et al. Interferon-gamma induces receptor dimerization in solution and on cells. J Biol Chem. 1993 Aug 25;268(24):18103-10. [Content Brief]

[4]. Akiyama K, et al. Replacement of proteasome subunits X and Y by LMP7 and LMP2 induced by interferon-gamma for acquirement of the functional diversity responsible for antigen processing. FEBS Lett. 1994 Apr 18;343(1):85-8. [Content Brief]

[5]. Fabunmi RP, et al. Interferon gamma regulates accumulation of the proteasome activator PA28 and immunoproteasomes at nuclear PML bodies. J Cell Sci. 2001 Jan;114(Pt 1):29-36. [Content Brief]

[6]. Lah TT, et al. Gamma-interferon causes a selective induction of the lysosomal proteases, cathepsins B and L, in macrophages. FEBS Lett. 1995 Apr 17;363(1-2):85-9. [Content Brief]

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IFN gamma Antibody (YA3520) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using IFN gamma Antibody (HY-P81111) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), Mouse spleen (lane 2, 15 μg), Rat spleen (lane 3, 15 μg), Mouse thymus (lane 4, 15 μg), and Rat thymus (lane 5, 15 μg) using IFN gamma Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • IFN gamma Antibody (YA3520) Product Image
    1/16
  • Experimental Validation Results for IFN gamma Antibody (YA3520)
  • Experimental Validation Results for IFN gamma Antibody (YA3520)
  • IFN gamma Antibody (YA3520) Product Image
  • Experimental Validation Results for IFN gamma Antibody (YA3520)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using IFN gamma Antibody (HY-P81111) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IFN gamma Antibody (YA3520)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), Mouse spleen (lane 2, 15 μg), Rat spleen (lane 3, 15 μg), Mouse thymus (lane 4, 15 μg), and Rat thymus (lane 5, 15 μg) using IFN gamma Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for IFN gamma Antibody (YA3520)
  • Experimental Validation Results for IFN gamma Antibody (YA3520)
  • IFN gamma Antibody (YA3520) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using IFN gamma Antibody (HY-P81111) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • IFN gamma Antibody (YA3520) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), Mouse spleen (lane 2, 15 μg), Rat spleen (lane 3, 15 μg), Mouse thymus (lane 4, 15 μg), and Rat thymus (lane 5, 15 μg) using IFN gamma Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • IFN gamma Antibody (YA3520) Product Image WB
  • IFN gamma Antibody (YA3520) Product Image WB