14-3-3 gamma Antibody (YA2668)(PBS only)

Cat. No.: HY-P82923A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

14-3-3 gamma Antibody (YA2668) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to 14-3-3 gamma.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

14-3-3 gamma Antibody (YA2668) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to 14-3-3 gamma.

Host

Rabbit

Molecular Weight

Predicted band size: 28 kDa;

Observed band size: 28 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P61981

Gene ID

7532 [NCBI]

Immunogen

A synthetic peptide of human 14-3-3 gamma/YWHAG

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:15696159, PubMed:16511572, PubMed:36732624). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:15696159, PubMed:16511572, PubMed:36732624). Binding generally results in the modulation of the activity of the binding partner (PubMed:16511572). Promotes inactivation of WDR24 component of the GATOR2 complex by binding to phosphorylated WDR24 (PubMed:36732624). Participates in the positive regulation of NMDA glutamate receptor activity by promoting the L-glutamate secretion through interaction with BEST1 (PubMed:29121962). Reduces keratinocyte intercellular adhesion, via interacting with PKP1 and sequestering it in the cytoplasm, thereby reducing its incorporation into desmosomes (PubMed:29678907). Plays a role in mitochondrial protein catabolic process (also named MALM) that promotes the degradation of damaged proteins inside mitochondria (PubMed:22532927)

Subcellular Localization：Cytoplasm, cytosol; Mitochondrion matrix

Expression：
Tissue_specificity:Highly expressed in brain, skeletal muscle, and heart

Subunit：Homodimer (PubMed:17085597). Part of a complex that contains DSG3, PKP1, YAP1 and YWHAG; the complex is required for localization of DSG3 and YAP1 to the cell membrane in keratinocytes (PubMed:31835537). Interacts with SAMSN1 (By similarity). Interacts with RAF1, SSH1 and CRTC2/TORC2 (PubMed:10433554, PubMed:15159416, PubMed:15454081). Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm (PubMed:15696159). Interacts with GAB2 (PubMed:19172738). Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53 (PubMed:16511572). Interacts with PKA-phosphorylated AANAT and SIRT2 (PubMed:11427721, PubMed:18249187). Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB, TBC1D22A and TBC1D22B (PubMed:23572552). Interacts with SLITRK1 (PubMed:19640509). Interacts with LRRK2; this interaction is dependent on LRRK2 phosphorylation (PubMed:28202711). Interacts with MARK2 and MARK3 (PubMed:16959763). Interacts with MEFV (PubMed:27030597). Interacts with ENDOG, TSC2 and PIK3C3; interaction with ENDOG weakens its interaction with TSC2 and PIK3C3 (PubMed:33473107). Interacts with (phosphorylated) WDR24 (PubMed:36732624). Interacts with BEST1; this interaction promotes L-glutamate channel activity leading to the positive regulation of NMDA glutamate receptor activity through the L-glutamate secretion (PubMed:29121962). Interacts with PKP1 (when phosphorylated); the interaction results in translocation of PKP1 to the cytoplasm and loss of intercellular adhesion in keratinocytes (PubMed:29678907). Interacts with SPATA18/MIEAP (isoforms 1 and 2); a protein that also plays a role in MALM (PubMed:22532927)

Research Field

Neuroscience

Synonyms

YWHAG; 14-3-3 protein gamma; Protein kinase C inhibitor protein 1; KCIP-1

Documentation

User Guide for Antibodies (1077 KB)