MyD88 Antibody (YA7452)

Cat. No.: HY-P87767: Data Sheet User Guide for Antibodies
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MyD88 Antibody (YA7452) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to MyD88.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation
References

Description

MyD88 Antibody (YA7452) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to MyD88.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 33 kDa;

Observed band size: 33 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q99836

Immunogen

The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000-1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000
IP IP: Immunoprecipitation	1:50-1:200

Sensitivity	Endogenous	Purity	Protein A affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG, Kappa

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from PC-12 (lane 1, 20μg), HepG2 (lane 2, 20μg), HCT116 (lane 3, 20μg), A594 (lane 4, 20μg), using MyD88 Antibody (YA7452) . Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 25°C for 1hour.The primary antibody (1:2000 dilution) and the loading control antibody (GAPDH, HY-P83944, 1:3000 dilution) were incubated in 5% non-fat milk in TBST for overnight. AF800-conjugated Goat Anti-Rabbit IgG H&L(HY-P810687, 1:1000 dilution) and AF680-conjugated Goat Anti-Mouse IgG H&L(HY-P89186 , 1:10000 dilution) was then applied for 1 hour at 37°C, protect from light.

Background

Function：Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response (PubMed:15361868, PubMed:18292575, PubMed:33718825, PubMed:37971847). Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:15361868, PubMed:19506249, PubMed:24316379). Increases IL-8 transcription (PubMed:9013863). Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Upon TLR8 activation by GU-rich single-stranded RNA (GU-rich RNA) derived from viruses such as SARS-CoV-2, SARS-CoV and HIV-1, induces IL1B release through NLRP3 inflammasome activation (PubMed:33718825). MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine (By similarity)

Subcellular Localization：Cytoplasm,Nucleus

Expression：
Tissue_Specificity: Ubiquitous

Isoforms & Post-Translational Modification：Q99836 has 6 isomers: Q99836-1: 33233 Da (predicted); Q99836-2: 28280 Da (predicted); Q99836-3: 20777 Da (predicted); Q99836-4: 15823 Da (predicted); Q99836-5: 31496 Da (predicted); Q99836-6: 34097 Da (predicted).
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination丨(Microbial infection) Ubiquitinated by human herpesvirus 8 (KSHV) protein RTA/ORF50, leading to proteasomal degradation ans suppression of TLR4 signaling pathway

Subunit：Homodimer

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/huvec-cell.html [Content Brief]