MyD88 Antibody (YA7452)
(Synonyms: Myeloid differentiation primary response protein MyD88, MYD88)MyD88 Antibody (YA7452) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to MyD88.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IP, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|
| Dilution Ratio | 1:2000-1:10000 | 1:200-1:1000 | 1:5000-1:20000 | 1:50-1:200 |
Product Details
MyD88 Antibody (YA7452) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to MyD88.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 33 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 33 kDa
The exact sequence is proprietary to MCE.
Endogenous
Protein A affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis was performed on extracts from PC-12 (lane 1, 20μg), HepG2 (lane 2, 20μg), HCT116 (lane 3, 20μg), A594 (lane 4, 20μg), using MyD88 Antibody (YA7452) . Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 25°C for 1hour.The primary antibody (1:2000 dilution) and the loading control antibody (GAPDH, HY-P83944, 1:3000 dilution) were incubated in 5% non-fat milk in TBST for overnight. AF800-conjugated Goat Anti-Rabbit IgG H&L(HY-P810687, 1:1000 dilution) and AF680-conjugated Goat Anti-Mouse IgG H&L(HY-P89186 , 1:10000 dilution) was then applied for 1 hour at 37°C, protect from light.
Background
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Function
MyD88 (Myeloid differentiation primary response gene 88) is a protein that, in humans, is encoded by the MYD88 gene. Available evidence suggests that MYD88 is dispensable for human resistance to common viral infections and to all but a few pyogenic bacterial infections, demonstrating a major difference between mouse and human immune responses. MyD88 is an essential adaptor protein in the IL-1R1 signaling pathway. MyD88 may define a family of signal transduction molecules with an ancestral function in the activation of the immune system. MyD88 functions as a pure adaptor linking the IL-1R1 to downstream IRAK kinases. Mutation in MYD88 at position 265 leading to a change from leucine to proline have been identified in many human lymphomas including ABC subtype of Diffuse Large B-cell Lymphoma and Waldenstrom's Macroglobulinemia.
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Subcellular Localization
Cytoplasm,Nucleus
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Expression
Tissue_Specificity: Ubiquitous -
Isoforms & Post-Translational Modification
Q99836 has 6 isomers: Q99836-1: 33233 Da (predicted); Q99836-2: 28280 Da (predicted); Q99836-3: 20777 Da (predicted); Q99836-4: 15823 Da (predicted); Q99836-5: 31496 Da (predicted); Q99836-6: 34097 Da (predicted).
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination丨(Microbial infection) Ubiquitinated by human herpesvirus 8 (KSHV) protein RTA/ORF50, leading to proteasomal degradation ans suppression of TLR4 signaling pathway -
Subunit
Homodimer
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SwissProt ID
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Synonyms
Myeloid differentiation primary response protein MyD88, MYD88
Documentation
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