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EIF2A Antibody (YA465)

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Based on 1 Customer Validation

EIF2A Antibody (YA465) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EIF2A.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for EIF2A Antibody (YA465)
  • EIF2A Antibody (YA465) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

EIF2A Antibody (YA465) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EIF2A.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 65 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 65 kDa
Species Reactivity Database

Entrez Gene: 83939 Human

SwissProt: Q9BY44 Human

Immunogen

Synthetic peptide corresponding to Human EIF2A.AA range:435-585.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102565

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EIF2A Antibody (YA465)
    Western blot analysis of extracts from Ramos (lane 1) and K562 (lane 2) and Hela (lane 3) and Raji (lane 4) using EIF2A antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner

  • Expression


    Tissue_specificity:Widely expressed. High expression levels are found in the pancreas, heart, brain, and placenta.

  • Isoforms & Post-Translational Modification

    Q9BY44 has 4 isomers: Q9BY44-1: 64990 Da (predicted); Q9BY44-2: 41339 Da (predicted); Q9BY44-3: 62289 Da (predicted); Q9BY44-4: 57994 Da (predicted).

  • SwissProt ID

    Q9BY44

  • Gene ID
    83939 [NCBI]
  • Research Field

    Epigenetics and Nuclear Signaling

EIF2A Antibody (YA465) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Ramos (lane 1) and K562 (lane 2) and Hela (lane 3) and Raji (lane 4) using EIF2A antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • EIF2A Antibody (YA465) Product Image
    1/16
  • Experimental Validation Results for EIF2A Antibody (YA465)
  • EIF2A Antibody (YA465) Product Image
  • Experimental Validation Results for EIF2A Antibody (YA465)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Ramos (lane 1) and K562 (lane 2) and Hela (lane 3) and Raji (lane 4) using EIF2A antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for EIF2A Antibody (YA465)
  • EIF2A Antibody (YA465) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Ramos (lane 1) and K562 (lane 2) and Hela (lane 3) and Raji (lane 4) using EIF2A antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • EIF2A Antibody (YA465) Product Image WB