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DDIT3 Antibody (YA7183)

(Synonyms: CHOP antibody,DDIT3 upstream open reading frame protein, AltDDIT3)
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Based on 1 Customer Validation

DDIT3 Antibody (YA7183) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DDIT3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for DDIT3 Antibody (YA7183)
  • Experimental Validation Results for DDIT3 Antibody (YA7183)
  • DDIT3 Antibody (YA7183) Product Image
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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IF/ICC/IF Info
 
FC Info
FC: Flow Cytometry
Dilution Ratio 1:5000 1:100 1:1000

Product Details

Description

DDIT3 Antibody (YA7183) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DDIT3.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 27 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 19 kDa,
Immunogen

Recombinant protein within human DDIT3 aa 1-169.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719309

Product Properties

  • Appearance

    Solution

  • Formulation

    supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Verification Images

  • Experimental Validation Results for DDIT3 Antibody (YA7183)
    Immunocytochemistry analysis of PC-3 cells labeling DDIT3 with DDIT3 Antibody (HY-P87495) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with DDIT3 Antibody (HY-P87495) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for DDIT3 Antibody (YA7183)
    Immunocytochemistry analysis of U2OS cells labeling DDIT3 with DDIT3 Antibody (HY-P87495) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with DDIT3 Antibody (HY-P87495) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    DDIT3 multifunctional transcription factor in endoplasmic reticulum (ER) stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Together with ATF4, mediates ER-mediated cell death by promoting expression of genes involved in cellular amino acid metabolic processes, mRNA translation and the unfolded protein response (UPR) in response to ER stress. Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response. Acts as a major regulator of postnatal neovascularization through regulation of endothelial nitric oxide synthase (NOS3)-related signaling[1][2][3][4][5][6][7][8][9].

  • Subcellular Localization

    Cytoplasm,Nucleus

  • Expression

    Induction: Up-regulated by oxidative stress, amino-acid deprivation, hypoxia and endoplasmic reticulum stress (PubMed:33384352). During ER stress, induced by a EIF2AK3/ATF4 pathway and/or ERN1/ATF6 pathway. Expression is suppressed by TLR-TRIF signaling pathway during prolonged ER stress

  • Isoforms & Post-Translational Modification

    P35638 has three isomers: P35638-1: 19175 Da (predicted); P35638-2: 21700 Da (predicted); P0DPQ6-1: 4284 Da (predicted).
    Ubiquitinated, leading to its degradation by the proteasome丨Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity

  • Subunit

    Heterodimer (PubMed:29083303)

  • SwissProt ID

    P35638

  • Synonyms

    CHOP antibody,DDIT3 upstream open reading frame protein, AltDDIT3

References

[1]. Yamaguchi H, et al. CHOP is involved in endoplasmic reticulum stress-induced apoptosis by enhancing DR5 expression in human carcinoma cells. J Biol Chem. 2004 Oct 29;279(44):45495-502. [Content Brief]

[2]. Ohoka N, et al. TRB3, a novel ER stress-inducible gene, is induced via ATF4-CHOP pathway and is involved in cell death. EMBO J. 2005 Mar 23;24(6):1243-55. [Content Brief]

[3]. Oliveira SJ, et al. ER stress-inducible factor CHOP affects the expression of hepcidin by modulating C/EBPalpha activity. PLoS One. 2009 Aug 12;4(8):e6618. [Content Brief]

[4]. Vij N, et al. CHOP transcription factor mediates IL-8 signaling in cystic fibrosis bronchial epithelial cells. Am J Respir Cell Mol Biol. 2008 Feb;38(2):176-84. [Content Brief]

[5]. Goodall JC, et al. Endoplasmic reticulum stress-induced transcription factor, CHOP, is crucial for dendritic cell IL-23 expression. Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17698-703. [Content Brief]

[6]. Ghosh AP, et al. CHOP potentially co-operates with FOXO3a in neuronal cells to regulate PUMA and BIM expression in response to ER stress. PLoS One. 2012;7(6):e39586. [Content Brief]

[7]. Su N, et al. C/EBP homology protein (CHOP) interacts with activating transcription factor 4 (ATF4) and negatively regulates the stress-dependent induction of the asparagine synthetase gene. J Biol Chem. 2008 Dec 12;283(50):35106-17. [Content Brief]

[8]. Park SH, et al. Endoplasmic reticulum stress-activated C/EBP homologous protein enhances nuclear factor-kappaB signals via repression of peroxisome proliferator-activated receptor gamma. J Biol Chem. 2010 Nov 12;285(46):35330-9. [Content Brief]

[9]. Horndasch M, et al. The C/EBP homologous protein CHOP (GADD153) is an inhibitor of Wnt/TCF signals. Oncogene. 2006 Jun 8;25(24):3397-407. [Content Brief]

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DDIT3 Antibody (YA7183) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling DDIT3 with DDIT3 Antibody (HY-P87495) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with DDIT3 Antibody (HY-P87495) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of U2OS cells labeling DDIT3 with DDIT3 Antibody (HY-P87495) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with DDIT3 Antibody (HY-P87495) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • DDIT3 Antibody (YA7183) Product Image
    1/16
  • Experimental Validation Results for DDIT3 Antibody (YA7183)
  • Experimental Validation Results for DDIT3 Antibody (YA7183)
  • DDIT3 Antibody (YA7183) Product Image
  • Experimental Validation Results for DDIT3 Antibody (YA7183)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling DDIT3 with DDIT3 Antibody (HY-P87495) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with DDIT3 Antibody (HY-P87495) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for DDIT3 Antibody (YA7183)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of U2OS cells labeling DDIT3 with DDIT3 Antibody (HY-P87495) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with DDIT3 Antibody (HY-P87495) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for DDIT3 Antibody (YA7183)
  • Experimental Validation Results for DDIT3 Antibody (YA7183)
  • DDIT3 Antibody (YA7183) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of PC-3 cells labeling DDIT3 with DDIT3 Antibody (HY-P87495) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with DDIT3 Antibody (HY-P87495) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • DDIT3 Antibody (YA7183) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of U2OS cells labeling DDIT3 with DDIT3 Antibody (HY-P87495) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with DDIT3 Antibody (HY-P87495) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • DDIT3 Antibody (YA7183) Product Image ICC
  • DDIT3 Antibody (YA7183) Product Image ICC