BRAT1 Antibody (YA5980)

Cat. No.: HY-P86288: Data Sheet User Guide for Antibodies
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BRAT1 Antibody (YA5980) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BRAT1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

BRAT1 Antibody (YA5980) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BRAT1.

Host

Rabbit

Clonality

Monoclonal

Molecular Weight

Observed band size: 90 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q6PJG6

Gene ID

221927 [NCBI]

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-5000
ICC	ICC/IF: 1:100-300
FC 1	100-300
IP IP: Immunoprecipitation	1:50-100

Purity	Protein A	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Component of a multiprotein complex required for the assembly of the RNA endonuclease module of the integrator complex (PubMed:39032489, PubMed:39032490). Associates with INTS9 and INTS11 in the cytoplasm and blocks the active site of INTS11 to inhibit the endonuclease activity of INTS11 before formation of the full integrator complex (PubMed:39032489, PubMed:39032490). Following dissociation of WDR73 of the complex, BRAT1 facilitates the nuclear import of the INTS9-INTS11 heterodimer (PubMed:39032489). In the nucleus, INTS4 is integrated to the INTS9-INTS11 heterodimer and BRAT1 is released from the mature RNA endonuclease module by inositol hexakisphosphate (InsP6) (PubMed:39032489). BRAT1 is also involved in DNA damage response; activates kinases ATM, SMC1A and PRKDC by modulating their phosphorylation status following ionizing radiation (IR) stress (PubMed:16452482, PubMed:22977523). Plays a role in regulating mitochondrial function and cell proliferation (PubMed:25070371). Required for protein stability of MTOR and MTOR-related proteins, and cell cycle progress by growth factors (PubMed:25657994)

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Tissue_specificity:general expression

Isoforms & Post-Translational Modification：Q6PJG6 has 3 isomers: Q6PJG6-1: 88119 Da (predicted); Q6PJG6-2: 30560 Da (predicted); Q6PJG6-3: 31676 Da (predicted).
Ubiquitinated by NEDD4, NEDD4L and ITCH; mono- and polyubiquitinated forms are detected

Subunit：Part of the multiprotein complex composed of BRAT1, WDR73, as well as integrator complex subunits INTS9 and INTS11 (PubMed:39032489). Interacts with BRCA1 and ATM (PubMed:16452482, PubMed:22977523). Interacts with MTOR and RPTOR (PubMed:25657994). Interacts with NDFIP1 (PubMed:25631046). Interacts with SMC1A and PRKDC (PubMed:22977523)

Synonyms

BRAT1; BAAT1; C7orf27; BRCA1-associated ATM activator 1 ; BRCA1-associated protein required for ATM activation protein 1;

Documentation

User Guide for Antibodies (1077 KB)