MLD Antibody (YA3235)
(Synonyms: MLD; DEGS; DES1; Des-1; FADS7; MIG15; DEGS-1)
Based on 1 Customer Validation
MLD Antibody (YA3235) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MLD.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
MLD Antibody (YA3235) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MLD.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 38 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 38 kDa
A synthetic peptide of human MLD
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from HEK293T(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using MLD Antibody (HY-P83490) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Background
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Function
MLD is a Has sphingolipid-delta-4-desaturase activity. Converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine). Catalyzes the equilibrium isomerization of retinols[1][2][3].
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Subcellular Localization
Mitochondrion membrane; Endoplasmic reticulum membrane; Multi-pass membrane protein
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Expression
Tissue_specificity:Ubiquitous -
Subunit
Interacts with RLBP1; the interaction increases synthesis of chromophore-precursors by DEGS1
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SwissProt ID
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Synonyms
MLD; DEGS; DES1; Des-1; FADS7; MIG15; DEGS-1
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Research Field
Cardiovascular
Documentation
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Data Sheet (260 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
[1]. Ternes P, et al. Identification and characterization of a sphingolipid delta 4-desaturase family. J Biol Chem. 2002 Jul 12;277(28):25512-8. [Content Brief]
[2]. Pant DC, et al. Loss of the sphingolipid desaturase DEGS1 causes hypomyelinating leukodystrophy. J Clin Invest. 2019 Mar 1;129(3):1240-1256. [Content Brief]
[3]. Karsai G, et al. DEGS1-associated aberrant sphingolipid metabolism impairs nervous system function in humans. J Clin Invest. 2019 Mar 1;129(3):1229-1239. [Content Brief]
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