2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. MLD Antibody (YA3235)

MLD Antibody (YA3235) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MLD.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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  • Description

Description

MLD Antibody (YA3235) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MLD.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Masse moléculaire
Predicted band size: 38 kDa;
Observed band size: 38 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human MLD
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Pureté Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Livraison

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from HEK293T(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using MLD Antibody (HY-P83490) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Has sphingolipid-delta-4-desaturase activity. Converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine) (PubMed:11937514, PubMed:30620337, PubMed:30620338). Catalyzes the equilibrium isomerization of retinols (By similarity)
Subcellular Localization：Mitochondrion membrane; Endoplasmic reticulum membrane; Multi-pass membrane protein
Expression：
Tissue_specificity:Ubiquitous
Subunit：Interacts with RLBP1; the interaction increases synthesis of chromophore-precursors by DEGS1
RRID
Database

Entrez Gene: 8560 Human

SwissProt: O15121 Human

OMIM: 618404 Human

Research Field

Cardiovascular
Synonyms
MLD; DEGS; DES1; Des-1; FADS7; MIG15; DEGS-1
Documentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

MLD Antibody (YA3235) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nom du produit:
MLD Antibody (YA3235)
Cat. No.:
HY-P83490
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