Phospho-Histone H2A.X (Ser139) Antibody (YA844)

Art. -Nr.: HY-P80941: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
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Phospho-Histone H2A.X (Ser139) Antibody (YA844) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Phospho-Histone H2A.X (Ser139).

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20 μL	Auf Lager	Estimated Time of Arrival: December 31
50 μL	Auf Lager	Estimated Time of Arrival: December 31
100 μL	Auf Lager	Estimated Time of Arrival: December 31
250 μL	Angebot einholen

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Phospho-Histone H2A.X (Ser139) Antibody (YA844) Spezialitätsempfehlungen:

1 Publications Citing Use of MCE Phospho-Histone H2A.X (Ser139) Antibody (YA844)

•Vet Parasitol. 2024 Jun 6:330:110217. [Abstract]

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung

Beschreibung

Phospho-Histone H2A.X (Ser139) Antibody (YA844) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Phospho-Histone H2A.X (Ser139).

Host

Mouse

Clonality

Monoclonal

Molekulargewicht

Predicted band size: 15 kDa;

Observed band size: 15 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P16104

Gene ID

3014 [NCBI]

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser139 of Human H2A.X.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Reinheit	Affinity Purified
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG1

Appearance

Liquid

Formulation

1.Supplied in PBS (pH7.3), 0.05% BSA and 50% Glycerol. Preservative: 0.02% Sodium Azide.
2.Supplied in 1xPBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA.
Please refer to the lot-specific COA for specific buffer information.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation

Subcellular Localization：Nucleus; Chromosome

Subunit：The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA (Probable). Interacts with numerous proteins required for DNA damage signaling and repair when phosphorylated on Ser-140 (PubMed:12419185, PubMed:12607005, PubMed:15201865). These include MDC1, TP53BP1, BRCA1 and the MRN complex, composed of MRE11, RAD50, and NBN (PubMed:12419185, PubMed:12607005, PubMed:15201865). Interaction with the MRN complex is mediated at least in part by NBN (PubMed:12419185). Also interacts with DHX9/NDHII when phosphorylated on Ser-140 and MCPH1 when phosphorylated at Ser-140 or Tyr-143 (PubMed:15613478). Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation (PubMed:16820410). Interacts with WRAP53/TCAB1 (PubMed:26734725, PubMed:27715493). Interacts with HDGFL2 (PubMed:26721387). Interacts with DNA damage up-regulated protein DDUP (PubMed:35849344). Forms a complex with DDUP and RAD18 following DDUP phosphorylation (PubMed:35849344)

RRID

AB_3102006

Database

Entrez Gene: 3014 Human ; 15270 Mouse ;

SwissProt: P16104 Human ; P27661 Mouse ;

OMIM: 601772 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X; gamma H2A.X

Dokumentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospho-Histone H2A.X (Ser139) Antibody (YA844) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-Histone H2A.X (Ser139) Antibody (YA844)

Phospho-Histone H2A.X (Ser139) Antibody (YA844) Spezialitätsempfehlungen:

1 Publications Citing Use of MCE Phospho-Histone H2A.X (Ser139) Antibody (YA844)

Product Detail

Background

Beschreibung

Phospho-Histone H2A.X (Ser139) Antibody (YA844) Related Classifications

Ihre kürzlich angesehenen Produkte:

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