Phospho-Histone H2A.X (Ser139) Antibody

Cat. No.: HY-P80941: FAQs
Data Sheet SDS COA User Guide for Antibodies

Phospho-Histone H2A.X (Ser139) Antibody is a non-conjugated and Mouse origined monoclonal antibody about 15 kDa, targeting to Histone H2AX. It can be used for WB,ICC/IF assays with N-6*His-tag, in the background of Human, Mouse.

For research use only. We do not sell to patients.

Size	Price	Stock	Quantity
10 μL	$90	In-stock	Estimated Time of Arrival: December 31
50 μL	$245	In-stock	Estimated Time of Arrival: December 31
100 μL	$400	In-stock	Estimated Time of Arrival: December 31
250 μL		Get quote

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Biological Activity
Technical Parameters
Properties
Documentation

Description	Phospho-Histone H2A.X (Ser139) Antibody is a non-conjugated and Mouse origined monoclonal antibody about 15 kDa, targeting to Histone H2AX. It can be used for WB,ICC/IF assays with N-6*His-tag, in the background of Human, Mouse.
Background	Histone H2AX: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]
Species	Virus
Tag	N-6*His
Gene ID	3014
Synonyms	H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X; gamma H2A.X
Molecular Weight	Predicted band size: 15 kDa
Purity	Affinity Purified
Conjugation	Non-conjugated
Research Field	Epigenetics and Nuclear Signaling
Product Categories	Primary Antibody; Mouse Monoclonal Antibody
Host	Mouse
Reactivity	Human, Mouse
SwissProt ID	P16104
Dilution Ratio	WB: 1:500-1:1,000 ;IF: 1:50-1:200
Application	WB, ICC/IF
Appearance	Liquid
Formulation	Supplied in PBS (pH7.3), 0.05% BSA and 50% Glycerol. Preservative: 0.02% Sodium Azide.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG1
Immunogen	Synthetic phosphopeptide corresponding to residues surrounding Ser139 of Human H2A.X.The exact sequence is proprietary to MCE.
Documentation	Select Batch: Data Sheet (234 KB) SDS (251 KB) COA (205 KB) User Guide for Antibodies (1077 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-Histone H2A.X (Ser139) Antibody

Biological Activity

Technical Parameters

Properties

Documentation

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