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Cat. No.: HY-P87582

ELP4 Antibody (YA7266)

ELP4 Antibody (YA7266) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ELP4.

For research use only. We do not sell to patients.

Formulation:
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications

Application	WB WB: Western Blot	IHC-P IHC-P: Immunohistochemistry-Paraffin	IHC-F IHC-F: Immunohistochemistry-Frozen	IF-Tissue IF-Tissue: Immunofluorescence-Tissue	ICC/IF ICC/IF: Immunocytochemistry/ Immunofluorescence
Dilution Ratio	1:1000-2000	1:100-200	1:100-200	1:50-200	1:50-200

Description

ELP4 Antibody (YA7266) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ELP4.

Host Rabbit
Clonality Recombinant,Monoclonal
Species Reactivity
Human Predicted Reactivity: Mouse

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
Observed Molecular Weight

Observed band size: 46 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 47 kDa

Immunogen

A synthesized peptide derived from human Elongator complex protein 4

Sensitivity

Endogenous

Purification

affinity purified by Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Appearance

Liquid
Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Function

ELP4 component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs^[1].
Subcellular Localization

Cytoplasm,Nucleus
Expression

Tissue_Specificity: Widely expressed
Isoforms & Post-Translational Modification

Q96EB1 has three isomers: Q96EB1-1: 46588 Da (predicted); Q96EB1-2: 58713 Da (predicted); Q96EB1-3: 52765 Da (predicted).
Subunit

Component of the elongator complex which consists of ELP1, ELP2, ELP3, ELP4, ELP5 and ELP6 (PubMed:11714725, PubMed:22854966)
SwissProt ID

Q96EB1