TREX1 Antibody (YA6812)

Cat. No.: HY-P87119: Data Sheet COA
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TREX1 Antibody (YA6812) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TREX1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

TREX1 Antibody (YA6812) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TREX1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 34 kDa;

Observed band size: 35 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Mouse

SwissProt ID

Q91XB0

Gene ID

22040 [NCBI]

Immunogen

Recombinant protein within mouse TREX1 aa 1-280.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000-1:5000
FC FC: Flow Cytometry	1:1000
IP IP: Immunoprecipitation	1-2μg/sample

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from MLTC-1(lane 2(40μg),Neuro-2a(lane 3(40μg), MC-38(lane 4(40μg),MS1(lane 5(40μg)using TREX1 Antibody (YA6812). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini (PubMed:10391904, PubMed:11279105, PubMed:15254239, PubMed:17293595, PubMed:17355961, PubMed:18780819). Prevents cell-intrinsic initiation of autoimmunity (PubMed:18724932, PubMed:24218451). Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements (PubMed:18724932). Plays a key role in degradation of DNA fragments at cytosolic micronuclei arising from genome instability: its association with the endoplasmic reticulum membrane directs TREX1 to ruptured micronuclei, leading to micronuclear DNA degradation (By similarity). Micronuclear DNA degradation is required to limit CGAS activation and subsequent inflammation (By similarity). Unless degraded, these DNA fragments accumulate in the cytosol and activate the cGAS-STING innate immune signaling, leading to the production of type I interferon (PubMed:18724932). Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates (PubMed:18045533). Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light (PubMed:23993650). During GZMA-mediated cell death, contributes to DNA damage in concert with NME1 (By similarity). NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair (By similarity)

Subcellular Localization：Nucleus,Cytoplasm, cytosol,Endoplasmic reticulum membrane

Expression：
Tissue_Specificity: Widely expressed with high expression levels detected in spleen, thymus and uterus
Induction: Induced by cytosolic DNA. Induced by inflammatory stimuli in a cell-specific fashion. Up-regulated by IFN-alpha and IFN-gamma in B-cells and by LPS and viral and bacterial DNA (via Toll-like receptor signaling) in dendritic cells and macrophages

Isoforms & Post-Translational Modification：Q91XB0: 314 amino acids, molecular weight 33675 Da.

Subunit：Homodimer (PubMed:17293595, PubMed:17355961, PubMed:22071149)

Synonyms

3' 5' exonuclease TREX1 antibody; 3' repair exonuclease 1 antibody; AGS1 antibody; AGS5 antibody; CRV antibody; Deoxyribonuclease III, dnaQ/mutD (E. coli) like antibody; DKFZp434J0310 antibody; DNase III antibody; DRN3 antibody; HERNS antibody; 3' 5' exonuclease TREX1 antibody; 3' repair exonuclease 1 antibody; AGS1 antibody; AGS5 antibody; CRV antibody; Deoxyribonuclease III, dnaQ/mutD (E. coli) like antibody; DKFZp434J0310 antibody; DNase III antibody; DRN3 antibody; HERNS antibody; Three prime repair exonuclease 1 antibody; TREX1 antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)