Copy Link Print Information

TREX1 Antibody (YA6812)

(Synonyms: 3' 5' exonuclease TREX1 antibody; 3' repair exonuclease 1 antibody; AGS1 antibody; AGS5 antibody; CRV antibody; Deoxyribonuclease III, dnaQ/mutD (E. coli) like antibody; DKFZp434J0310 antibody; DNase III antibody; DRN3 antibody; HERNS antibody; 3' 5' exonuclease TREX1 antibody; 3' repair exonuclease 1 antibody; AGS1 antibody; AGS5 antibody; CRV antibody; Deoxyribonuclease III, dnaQ/mutD (E. coli) like antibody; DKFZp434J0310 antibody; DNase III antibody; DRN3 antibody; HERNS antibody; Three prime repair exonuclease 1 antibody; TREX1 antibody; )
Customer Review

Based on 1 Customer Validation

TREX1 Antibody (YA6812) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TREX1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC, IP

  • Reactivity :

    Mouse

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TREX1 Antibody (YA6812)
  • TREX1 Antibody (YA6812) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000-1:5000 1:1000 1-2μg/sample

Product Details

Description

TREX1 Antibody (YA6812) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TREX1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Mouse
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 34 kDa
Immunogen

Recombinant protein within mouse TREX1 aa 1-280.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TREX1 Antibody (YA6812)
    Western blot analysis of extracts from MLTC-1(lane 2(40μg),Neuro-2a(lane 3(40μg), MC-38(lane 4(40μg),MS1(lane 5(40μg)using TREX1 Antibody (YA6812). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    TREX1 is a Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini. Prevents cell-intrinsic initiation of autoimmunity. Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements. Plays a key role in degradation of DNA fragments at cytosolic micronuclei arising from genome instability: its association with the endoplasmic reticulum membrane directs TREX1 to ruptured micronuclei, leading to micronuclear DNA degradation. Micronuclear DNA degradation is required to limit CGAS activation and subsequent inflammation. Unless degraded, these DNA fragments accumulate in the cytosol and activate the cGAS-STING innate immune signaling, leading to the production of type I interferon. Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates. Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light. During GZMA-mediated cell death, contributes to DNA damage in concert with NME1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair[1][2][3][4][5][6][7][8][9][10].

  • Subcellular Localization

    Nucleus,Cytoplasm, cytosol,Endoplasmic reticulum membrane

  • Expression


    Tissue_Specificity: Widely expressed with high expression levels detected in spleen, thymus and uterus
    Induction: Induced by cytosolic DNA. Induced by inflammatory stimuli in a cell-specific fashion. Up-regulated by IFN-alpha and IFN-gamma in B-cells and by LPS and viral and bacterial DNA (via Toll-like receptor signaling) in dendritic cells and macrophages

  • Isoforms & Post-Translational Modification

    Q91XB0: 314 amino acids, molecular weight 33675 Da.

  • Subunit

    Homodimer (PubMed:17293595, PubMed:17355961, PubMed:22071149)

  • SwissProt ID

    Q91XB0

  • Gene ID
    22040 [NCBI]
  • Synonyms

    3' 5' exonuclease TREX1 antibody; 3' repair exonuclease 1 antibody; AGS1 antibody; AGS5 antibody; CRV antibody; Deoxyribonuclease III, dnaQ/mutD (E. coli) like antibody; DKFZp434J0310 antibody; DNase III antibody; DRN3 antibody; HERNS antibody; 3' 5' exonuclease TREX1 antibody; 3' repair exonuclease 1 antibody; AGS1 antibody; AGS5 antibody; CRV antibody; Deoxyribonuclease III, dnaQ/mutD (E. coli) like antibody; DKFZp434J0310 antibody; DNase III antibody; DRN3 antibody; HERNS antibody; Three prime repair exonuclease 1 antibody; TREX1 antibody;

TREX1 Antibody (YA6812) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MLTC-1(lane 2(40μg),Neuro-2a(lane 3(40μg), MC-38(lane 4(40μg),MS1(lane 5(40μg)using TREX1 Antibody (YA6812). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • TREX1 Antibody (YA6812) Product Image
    1/16
  • Experimental Validation Results for TREX1 Antibody (YA6812)
  • TREX1 Antibody (YA6812) Product Image
  • Experimental Validation Results for TREX1 Antibody (YA6812)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MLTC-1(lane 2(40μg),Neuro-2a(lane 3(40μg), MC-38(lane 4(40μg),MS1(lane 5(40μg)using TREX1 Antibody (YA6812). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TREX1 Antibody (YA6812)
  • TREX1 Antibody (YA6812) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MLTC-1(lane 2(40μg),Neuro-2a(lane 3(40μg), MC-38(lane 4(40μg),MS1(lane 5(40μg)using TREX1 Antibody (YA6812). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • TREX1 Antibody (YA6812) Product Image WB