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HRP-conjugated Anti-Mouse lgG for IP

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HRP-conjugated Anti-Mouse lgG for IP is an HRP-conjugated,Rat-derived anti-Mouse antibody. HRP-conjugated Anti-Mouse lgG for IP can binds to the IgG heavy and light chain of Mouse antibodies.Specifically designed for IP、Western blotting, minimizes detection of denatured mouse IgG heavy (55 kDa) and light (25 kDa) chains, reducing background and improving sensitivity for low-abundance proteins and targets near 25 kDa or 55 kDa.

For research use only. We do not sell to patients.
  • Host:

    Rat

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, mIHC, ELISA

  • Reactivity :

    Mouse

  • Formulation:

    Supplied in PBS, PH7.4.

  • Conjugation:
    HRP
  • Experimental Validation Results for HRP-conjugated Anti-Mouse lgG for IP
  • HRP-conjugated Anti-Mouse lgG for IP Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
mIHC Info
mIHC: Multiplex Immunohistochemical
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:5000-1:20000 1:500-1:1000 1:500-1000 1:5000-1:20000

Product Details

Description

HRP-conjugated Anti-Mouse lgG for IP is an HRP-conjugated,Rat-derived anti-Mouse antibody. HRP-conjugated Anti-Mouse lgG for IP can binds to the IgG heavy and light chain of Mouse antibodies.Specifically designed for IP、Western blotting, minimizes detection of denatured mouse IgG heavy (55 kDa) and light (25 kDa) chains, reducing background and improving sensitivity for low-abundance proteins and targets near 25 kDa or 55 kDa.

  • Host Rat
  • Clonality Monoclonal
  • Species Reactivity
    Mouse
Immunogen

Mouse IgG

Purification

Affinity Purified

Conjugation

HRP

Modification

Unmodified

Target Specificity

IgG heavy and light chain

Antibody Form

Whole IgG Antibodies

Isotype

IgG

RRID

AB_3719311

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, PH7.4.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HRP-conjugated Anti-Mouse lgG for IP
    Western blot analysis was performed on extracts from Mouse mAb(Boiled/Reduced) (lane 1, 10 ng), Mouse IgG(Boiled/Reduced) (lane 2, 10 ng), Mouse mAb(Unboiled/Non-reduced) (lane 3, 10 ng), and Mouse IgG(Unboiled/Non-reduced) (lane 4, 10 ng) using ExactBlot™ Anti-Mouse lgG for IP(HRP). Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

Background

  • Function

    Secondary antibodies are antibodies that bind to primary antibodies in immunoassays. They do not directly recognize the target antigen but rather the Fc region of the primary antibody or the entire immunoglobulin (Ig). Secondary antibodies are typically conjugated to fluorescent groups (such as Alexa Fluor), enzymes (such as HRP and alkaline phosphatase), or proteins (such as biotin).

  • Research Field

    Immunology

HRP-conjugated Anti-Mouse lgG for IP Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Mouse mAb(Boiled/Reduced) (lane 1, 10 ng), Mouse IgG(Boiled/Reduced) (lane 2, 10 ng), Mouse mAb(Unboiled/Non-reduced) (lane 3, 10 ng), and Mouse IgG(Unboiled/Non-reduced) (lane 4, 10 ng) using ExactBlot™ Anti-Mouse lgG for IP(HRP). Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

  • HRP-conjugated Anti-Mouse lgG for IP Product Image
    1/16
  • Experimental Validation Results for HRP-conjugated Anti-Mouse lgG for IP
  • HRP-conjugated Anti-Mouse lgG for IP Product Image
  • Experimental Validation Results for HRP-conjugated Anti-Mouse lgG for IP
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Mouse mAb(Boiled/Reduced) (lane 1, 10 ng), Mouse IgG(Boiled/Reduced) (lane 2, 10 ng), Mouse mAb(Unboiled/Non-reduced) (lane 3, 10 ng), and Mouse IgG(Unboiled/Non-reduced) (lane 4, 10 ng) using ExactBlot™ Anti-Mouse lgG for IP(HRP). Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.
  • Experimental Validation Results for HRP-conjugated Anti-Mouse lgG for IP
  • HRP-conjugated Anti-Mouse lgG for IP Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Mouse mAb(Boiled/Reduced) (lane 1, 10 ng), Mouse IgG(Boiled/Reduced) (lane 2, 10 ng), Mouse mAb(Unboiled/Non-reduced) (lane 3, 10 ng), and Mouse IgG(Unboiled/Non-reduced) (lane 4, 10 ng) using ExactBlot™ Anti-Mouse lgG for IP(HRP). Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

  • HRP-conjugated Anti-Mouse lgG for IP Product Image WB