HRP-conjugated Anti-Mouse lgG for IP

Cat. No.: HY-P87497: Data Sheet COA
SDS
User Guide for Antibodies
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HRP-conjugated Anti-Mouse lgG is a conformation-specific secondary antibody that binds exclusively to native, non-denatured IgG and does not recognize denatured IgG. Conformation-specific secondary antibodies target spatial epitopes. When antibodies are denatured by high temperatures, they lose their native conformation and are no longer recognized by such antibodies—neither the heavy chain nor the light chain is detected. This effectively avoids interference from the heavy chain (55 kDa) and light chain (25 kDa) in immunoprecipitation (IP) experiments.

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사이즈	재고	수량
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

1 Publications Citing Use of MCE HRP-conjugated Anti-Mouse lgG for IP

•Res Sq. 2026 Mar 3.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
제품 설명

제품 설명

Host

Rat

Clonality

Monoclonal

Species Reactivity

Mouse

Immunogen

Mouse IgG

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2500-1:10000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:1000-1:3000

Conjugation	HRP	Modification	Unmodified

Appearance

Solution

Formulation

Liquid in phosphate Buffered Saline containing 1% BSA, 0.01% thimerosal, 50% glycerol, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Mouse mAb(Boiled/Reduced) (lane 1, 10 ng), Mouse IgG(Boiled/Reduced) (lane 2, 10 ng), Mouse mAb(Unboiled/Non-reduced) (lane 3, 10 ng), and Mouse IgG(Unboiled/Non-reduced) (lane 4, 10 ng) using ExactBlot™ Anti-Mouse lgG for IP(HRP). Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.

RRID

AB_3719311

Research Field

Immunology

각종 서류

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Data Sheet (231 KB) SDS (251 KB)

COA (204 KB)

User Guide for Antibodies (1077 KB)