PTEN Antibody (YA4114)(PBS only)
(Synonyms: BZS; MHAM; TEP1; PTEN1)PTEN Antibody (YA4114) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PTEN.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, FC, ELISA
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
PTEN Antibody (YA4114) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PTEN.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 54 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 47 kDa
Purified recombinant fragment of PTEN full(aa 1-403).
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PTEN (Phosphatase and tensin homologue deleted on chromosome 10), a phosphoinositide 3-phosphatase, is an important regulator of insulin-dependent signaling. The loss or impairment of PTEN results in an antidiabetic impact, which led to the suggestion that PTEN could be an important target for drugs against type II diabetes. PTEN has a much wider active site cleft enabling it to bind the PtdIns (3, 4, 5) P3 substrate. a highly potent and specific inhibitor of PTEN that increases cellular PtdIns (3, 4, 5) P3 levels, phosphorylation of Akt, and glucose uptake in adipocytes at nanomolar concentrations.
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Subcellular Localization
Cytoplasm; Nucleus; Nucleus, PML body; Cell projection, dendritic spine; Postsynaptic density; Secreted
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Expression
Tissue_specificity:It is expressed at high levels in all adult tissues, including the heart, brain, placenta, lungs, liver, muscles, kidneys, and pancreas.
Induction:Down-regulated by TGFB1 -
Subunit
Monomer. The unphosphorylated form interacts with the second PDZ domain of MAGI2 and with DLG1 and MAST2 in vitro (PubMed:10646847, PubMed:10760291, PubMed:11707428). Interacts with MAGI2, MAGI3, MAST1 and MAST3, but neither with MAST4 nor with DLG5; interaction with MAGI2 increases protein stability (PubMed:10748157, PubMed:15951562). Interacts with NEDD4 (PubMed:17218260). Interacts with NDFIP1 and NDFIP2; in the presence of NEDD4 or ITCH, this interaction promotes PTEN ubiquitination (PubMed:20534535, PubMed:25801959). Interacts (via C2 domain) with FRK (PubMed:19345329). Interacts with USP7; the interaction is direct (PubMed:18716620). Interacts with ROCK1 (By similarity). Interacts with XIAP/BIRC4 (PubMed:19473982). Interacts with STK11; the interaction phosphorylates PTEN (PubMed:15987703). Interacts with PPP1R16B (PubMed:25007873). Interacts with NOP53; regulates PTEN phosphorylation and increases its stability (PubMed:15355975). Interacts (via PDZ domain-binding motif) with DLG4; the interaction is induced by NMDA and is required for PTEN location at postsynaptic density (By similarity). Interacts with the regulatory p85 subunit of the PI3K kinase complex and with Rho GTPase-activating protein DLC1; in resting cells, interacts (via C2 tensin-type domain) with p85 but, following growth factor stimulation, PTEN is phosphorylated which leads to weakened interaction with p85 and enhanced interaction (via C2 tensin-type domain) with DLC1 while p85 interaction with TNS3 increases (PubMed:26166433)
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SwissProt ID
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Synonyms
BZS; MHAM; TEP1; PTEN1
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