Copy Link Print Information

Phospho-Moesin (Thr558) Antibody (YA2614)

(Synonyms: MSN; Moesin; Membrane-organizing extension spike protein; RDX; Radixin; EZR; VIL2; Ezrin; Cytovillin; Villin-2; p81)
Customer Review

Based on 1 Customer Validation

Phospho-Moesin (Thr558) Antibody (YA2614) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Moesin (Thr558).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-Moesin (Thr558) Antibody (YA2614)
  • Phospho-Moesin (Thr558) Antibody (YA2614) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Phospho-Moesin (Thr558) Antibody (YA2614) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Moesin (Thr558).

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 68 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 68 kDa
Species Reactivity Database
Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Thr558 of human Moesin

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3104962

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-Moesin (Thr558) Antibody (YA2614)
    Western blot analysis of extracts from Hela (lane2, 20μg), A549 (lane3, 20μg) and HL-60 (lane4, 20μg) using Phospho-Moesin (Thr558) Antibody (HY-P82869). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Moesin is an Ezrin-radixin-moesin (ERM) family protein that connects the actin cytoskeleton to the plasma membrane and thereby regulates the structure and function of specific domains of the cell cortex. Tethers actin filaments by oscillating between a resting and an activated state providing transient interactions between moesin and the actin cytoskeleton. Once phosphorylated on its C-terminal threonine, moesin is activated leading to interaction with F-actin and cytoskeletal rearrangement. These rearrangements regulate many cellular processes, including cell shape determination, membrane transport, and signal transduction. The role of moesin is particularly important in immunity acting on both T and B-cells homeostasis and self-tolerance, regulating lymphocyte egress from lymphoid organs. Modulates phagolysosomal biogenesis in macrophages. Also participates in immunologic synapse formation[1][2][3][4][5][6].

  • Subcellular Localization

    Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cytoplasm, cytoskeleton; Apical cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, microvillus membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, microvillus

  • Expression


    Tissue_specificity:In all the tissues and cultured cells studied

  • Subunit

    In resting T-cells, part of a PAG1-NHERF1-MSN complex which is disrupted upon TCR activation. Interacts with NHERF1 (PubMed:15020681, PubMed:9314537). Interacts with PPP1R16B (PubMed:18586956). Interacts with SELPLG and SYK; these interactions mediate the activation of SYK by SELPLG (PubMed:12387735). Interacts with PDPN (via cytoplasmic domain); this interaction activates RHOA and promotes epithelial-mesenchymal transition (PubMed:17046996). Interacts with SPN/CD43 cytoplasmic tail (PubMed:11728332, PubMed:9616160). Interacts with CD44 (PubMed:9298994). Interacts with ICAM2 (By similarity). Interacts with ICAM3 (via C-terminus) (PubMed:9298994). Interacts with PDZD8 (PubMed:21549406). Interacts with F-actin (PubMed:10212266). Interacts with CD46 (PubMed:7884872). Interacts with PTPN6 (By similarity)

  • SwissProt ID

    P26038

  • Gene ID
    4478 [NCBI]
  • Synonyms

    MSN; Moesin; Membrane-organizing extension spike protein; RDX; Radixin; EZR; VIL2; Ezrin; Cytovillin; Villin-2; p81

  • Research Field

    Signal Transduction

Phospho-Moesin (Thr558) Antibody (YA2614) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), A549 (lane3, 20μg) and HL-60 (lane4, 20μg) using Phospho-Moesin (Thr558) Antibody (HY-P82869). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Phospho-Moesin (Thr558) Antibody (YA2614) Product Image
    1/16
  • Experimental Validation Results for Phospho-Moesin (Thr558) Antibody (YA2614)
  • Phospho-Moesin (Thr558) Antibody (YA2614) Product Image
  • Experimental Validation Results for Phospho-Moesin (Thr558) Antibody (YA2614)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), A549 (lane3, 20μg) and HL-60 (lane4, 20μg) using Phospho-Moesin (Thr558) Antibody (HY-P82869). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-Moesin (Thr558) Antibody (YA2614)
  • Phospho-Moesin (Thr558) Antibody (YA2614) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2, 20μg), A549 (lane3, 20μg) and HL-60 (lane4, 20μg) using Phospho-Moesin (Thr558) Antibody (HY-P82869). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Phospho-Moesin (Thr558) Antibody (YA2614) Product Image WB