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Ly6g Antibody (YA6106)

(Synonyms: Gr-1; Gr1; Ly-6G.1; Ly6G; Lymphocyte antigen 6 complex locus G; Lymphocyte antigen 6G)
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Based on 1 publication(s) in Google Scholar

Ly6g Antibody (YA6106) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ly6g.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, ELISA

  • Reactivity :

    Mouse

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for Ly6g Antibody (YA6106)
  • Experimental Validation Results for Ly6g Antibody (YA6106)
  • Experimental Validation Results for Ly6g Antibody (YA6106)
  • Experimental Validation Results for Ly6g Antibody (YA6106)
  • Experimental Validation Results for Ly6g Antibody (YA6106)
  • Ly6g Antibody (YA6106) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:1000-1:4000 1:1000-1:5000 1:200-1:1000 1:5000-1:20000

Product Details

Description

Ly6g Antibody (YA6106) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ly6g.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Mouse
  • Observed Molecular Weight
    Observed band size: 14 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 14 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719150

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Ly6g Antibody (YA6106)
    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Ly6g Antibody (YA6106)
    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Ly6g Antibody (YA6106)
    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Ly6g Antibody (YA6106)
    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Ly6g Antibody (YA6106)
    Immunohistochemical analysis of paraffin-embedded Mouse subcutaneous tumor tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

Background

  • Function

    Ly-6G is the most classic surface marker for mouse neutrophils and is a GPI-anchored protein belonging to the Ly-6/uPAR family. Ly-6G is primarily used in flow cytometry to identify neutrophils, analyze inflammatory cell infiltration, and study tumor-associated neutrophils and PMN-MDSCs; it is also commonly employed in experiments involving anti-Ly-6G antibody-mediated neutrophil depletion in mice.

  • Subcellular Localization

    Cell membrane; Lipid-anchor, GPI-anchor

  • Expression


    Tissue_specificity:Expressed in bone marrow

  • SwissProt ID

    P35461

  • Gene ID
    546644 [NCBI]
  • Synonyms

    Gr-1; Gr1; Ly-6G.1; Ly6G; Lymphocyte antigen 6 complex locus G; Lymphocyte antigen 6G

Ly6g Antibody (YA6106) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse subcutaneous tumor tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Ly6g Antibody (YA6106) Product Image
    1/16
  • Experimental Validation Results for Ly6g Antibody (YA6106)
  • Experimental Validation Results for Ly6g Antibody (YA6106)
  • Experimental Validation Results for Ly6g Antibody (YA6106)
  • Experimental Validation Results for Ly6g Antibody (YA6106)
  • Experimental Validation Results for Ly6g Antibody (YA6106)
  • Ly6g Antibody (YA6106) Product Image
  • Experimental Validation Results for Ly6g Antibody (YA6106)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Ly6g Antibody (YA6106)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Ly6g Antibody (YA6106)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Ly6g Antibody (YA6106)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Ly6g Antibody (YA6106)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse subcutaneous tumor tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Ly6g Antibody (YA6106)
  • Experimental Validation Results for Ly6g Antibody (YA6106)
  • Experimental Validation Results for Ly6g Antibody (YA6106)
  • Experimental Validation Results for Ly6g Antibody (YA6106)
  • Experimental Validation Results for Ly6g Antibody (YA6106)
  • Ly6g Antibody (YA6106) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Ly6g Antibody (YA6106) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Ly6g Antibody (YA6106) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Ly6g Antibody (YA6106) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Ly6g Antibody (YA6106) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse subcutaneous tumor tissue using Ly6g Antibody (YA6106). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86414, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Ly6g Antibody (YA6106) Product Image IHC-P
  • Ly6g Antibody (YA6106) Product Image IHC-P
  • Ly6g Antibody (YA6106) Product Image IHC-P
  • Ly6g Antibody (YA6106) Product Image IHC-P
  • Ly6g Antibody (YA6106) Product Image IHC-P