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BSA Antibody (YA4759)

Customer Review

Based on 1 Customer Validation

BSA Antibody (YA4759) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to BSA.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Application:

    WB, ELISA

  • Reactivity :

    Bovine

  • Formulation:

    Supplied in PBS containing 50% glycerol,0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for BSA Antibody (YA4759)
  • BSA Antibody (YA4759) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:10000

Product Details

Description

BSA Antibody (YA4759) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to BSA.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Bovine
Immunogen

Bovine serum albumin.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol,0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for BSA Antibody (YA4759)
    Western blot analysis was performed by loading 1 μg (Lane 2), 2 μg (Lane 3), 2.5 μg (Lane 4) and 5 μg (Lane 5) of BSA using BSA antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    BSA binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (Probable). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner. The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (Probable). The rank order of affinity is zinc > calcium > magnesium (Probable). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growtH2O2 enteric bacteria such as E.coli. Does not prevent iron uptake by the bacterial siderophore aerobactin[1][2].

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:plasma

  • Subunit

    Interacts with FCGRT; this interaction regulates ALB homeostasis (By similarity). Interacts with TASOR (By similarity).

  • SwissProt ID

    P02769

  • Gene ID
    280717 [NCBI]

BSA Antibody (YA4759) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed by loading 1 μg (Lane 2), 2 μg (Lane 3), 2.5 μg (Lane 4) and 5 μg (Lane 5) of BSA using BSA antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • BSA Antibody (YA4759) Product Image
    1/16
  • Experimental Validation Results for BSA Antibody (YA4759)
  • BSA Antibody (YA4759) Product Image
  • Experimental Validation Results for BSA Antibody (YA4759)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed by loading 1 μg (Lane 2), 2 μg (Lane 3), 2.5 μg (Lane 4) and 5 μg (Lane 5) of BSA using BSA antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for BSA Antibody (YA4759)
  • BSA Antibody (YA4759) Product Image
    MedChemExpress Validation

    Western blot analysis was performed by loading 1 μg (Lane 2), 2 μg (Lane 3), 2.5 μg (Lane 4) and 5 μg (Lane 5) of BSA using BSA antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • BSA Antibody (YA4759) Product Image WB