Securin Antibody (YA1917)(PBS only)

Cat. No.: HY-P82172A: Data Sheet User Guide for Antibodies
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Securin Antibody (YA1917) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Securin.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

Securin Antibody (YA1917) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Securin.

Host

Rabbit

Molecular Weight

Predicted band size: 22 kDa;

Observed band size: 25 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O95997

Gene ID

9232 [NCBI]

Immunogen

A synthetic peptide of human Securin

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:20-1:50
FC FC: Flow Cytometry	1:20-1:50

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer

Subunit：Interacts with the caspase-like ESPL1, and prevents its protease activity probably by covering its active site. Interacts with TP53 and blocks its activity probably by blocking its binding to DNA.

Research Field

Cell Biology

Synonyms

PTTG1; EAP1; PTTG; TUTR1; Securin; Esp1-associated protein; Pituitary tumor-transforming gene 1 protein; Tumor-transforming protein 1; hPTTG

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/nci-h460-cell.html [Content Brief]