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LHX2 Antibody (YA1973)

(Synonyms: LHX2; LH2; LIM/homeobox protein Lhx2; Homeobox protein LH-2; LIM homeobox protein 2)
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Based on 1 Customer Validation

LHX2 Antibody (YA1973) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LHX2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for LHX2 Antibody (YA1973)
  • LHX2 Antibody (YA1973) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

LHX2 Antibody (YA1973) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LHX2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 44 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 44 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human LHX2 aa1-56/406.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104341

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for LHX2 Antibody (YA1973)
    Western blot analysis was performed on extracts from A549 (lane 1, 20 μg) using LHX2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    LHX2 acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types

  • Subcellular Localization

    Nucleus

  • Subunit

    Interacts (via LIM domains) with CITED2. Interacts with POU4F2

  • SwissProt ID

    P50458

  • Gene ID
    9355 [NCBI]
  • Synonyms

    LHX2; LH2; LIM/homeobox protein Lhx2; Homeobox protein LH-2; LIM homeobox protein 2

  • Research Field

    Neuroscience

LHX2 Antibody (YA1973) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from A549 (lane 1, 20 μg) using LHX2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • LHX2 Antibody (YA1973) Product Image
    1/16
  • Experimental Validation Results for LHX2 Antibody (YA1973)
  • LHX2 Antibody (YA1973) Product Image
  • Experimental Validation Results for LHX2 Antibody (YA1973)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from A549 (lane 1, 20 μg) using LHX2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for LHX2 Antibody (YA1973)
  • LHX2 Antibody (YA1973) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from A549 (lane 1, 20 μg) using LHX2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • LHX2 Antibody (YA1973) Product Image WB